In realizing the vast potential of lignocellulosic biomass residues generated by oil palm industry under the regime of ‘one-size-fits-all’ biorefinery concept, supercritical carbon dioxide (SC-CO₂) technology offers great promise. Herein, we demonstrated a new eco-friendly pretreatment of oil palm trunk biomass based on SC-CO₂ under controlled conditions of pressure (25, 35 and 45 MPa), temperature (80, 100 and 120 °C) and moisture content (0, 20, 40 and 60 %), and investigated their effect on enzymatic hydrolysis yields of the pretreated fractions. During pretreatment, biomass sample reacted with hot water in SC-CO₂ which led to high diffusivities, promoting its disruption and xylan solubilisation as supported by various characterization techniques. This study illustrates that hydrolysis yields are influenced by process parameters, particularly the moisture content played a crucial role in the biomass disruption. The SC-CO₂ pretreatment resulted in enhanced digestibility for all the pretreated fractions compared to the untreated biomass.

超临界二氧化碳（SC-CO ₂）技术在实现“一刀切”生物精炼概念下由油棕工业产生的木质纤维素生物质残余物的巨大潜力中。本文中，我们展示了在压力（25、35和45 MPa），温度（80、100和120°C）和水分（0的控制条件下，基于SC-CO _2的油棕树干生物质的新型环保预处理方法（分别为20％，40％和60％），并研究了它们对预处理馏分的酶水解产率的影响。在预处理期间，生物质样品与SC-CO _2中的热水反应这导致了高扩散性，并在各种表征技术的支持下促进了它的破坏和木聚糖的溶解。这项研究表明，水解产量受工艺参数的影响，尤其是水分含量在生物质破坏中起着至关重要的作用。与未经处理的生物质相比，SC-CO ₂预处理可提高所有预处理馏分的消化率。