The paper presents an influence of the surface mechanical properties of thin-film materials on blood cell adhesion under shear stress conditions. Physical vapour deposited (PVD) coatings i.e. hydrogenated amorphous carbon (a-C:H) doped with nitrogen or silicon have been investigated. The mechanical properties of materials, namely their microhardness and Young’s modulus were measured using indentation test with Rockwell indenter. The adhesion efficiency of blood cells in dynamic conditions were analysed using a radial flow chamber. Red blood cells (RBC) were used as representative cells to analyse cell–material interactions. The biomaterial examinations were performed under physiological flow conditions at the single-cell level. The 3D FVM (finite volume method) model of multi-phase radial flow test was developed to reproduce the physical test and to predict distributions of shear stresses and velocity during blood washout with PBS. Cell–material interactions were found to be strongly associated with the mechanical properties of the thin-film material. The decrease in the hardness of the coatings translated into a weaker cell – material interactions.

本文提出了在剪切应力条件下薄膜材料的表面力学性能对血细胞粘附的影响。已经研究了物理气相沉积（PVD）涂层，即掺杂有氮或硅的氢化非晶碳（aC：H）。材料的机械性能，即它们的显微硬度和杨氏模量是使用Rockwell压头进行压痕测试来测量的。使用径向流动室分析动态条件下血细胞的粘附效率。红细胞（RBC）被用作代表细胞来分析细胞与物质的相互作用。生物材料检查是在生理流动条件下在单细胞水平上进行的。建立了多相径向流测试的3D FVM（有限体积法）模型，以再现物理测试并预测在用PBS进行血液冲洗时的剪切应力和速度分布。发现细胞与材料的相互作用与薄膜材料的机械性能密切相关。涂层硬度的降低转化为较弱的单元-材料相互作用。