Detection of antibodies to upper respiratory pathogens is critical to surveillance, assessment of the immune status of individuals, vaccine development, and basic biology. The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. We report a multiplex label-free antigen microarray on the Arrayed Imaging Reflectometry (AIR) platform for detection of antibodies to SARS-CoV-2, SARS-CoV-1, MERS, three circulating coronavirus strains (HKU1, 229E, OC43) and three strains of influenza. We find that the array is readily able to distinguish uninfected from convalescent COVID-19 subjects, and provides quantitative information about total Ig, as well as IgG- and IgM-specific responses.

上呼吸道病原体抗体的检测对于监测、个体免疫状态评估、疫苗开发和基础生物学至关重要。事实证明，在 COVID-19 时代，对抗体检测工具的迫切需求尤为迫切。我们在阵列成像反射计 (AIR) 平台上报告了一种多重无标记抗原微阵列，用于检测 SARS-CoV-2、SARS-CoV-1、MERS、三种流行冠状病毒株（HKU1、229E、OC43）和三种病毒的抗体。流感病毒株。我们发现该芯片能够轻松区分未感染者和恢复期的 COVID-19 受试者，并提供有关总 Ig 以及 IgG 和 IgM 特异性反应的定量信息。