Genes & Genomics ( IF 2.1 ) Pub Date : 2020-09-21 , DOI: 10.1007/s13258-020-00995-9 Shuang Liang 1 , Zhiyuan Bao 1 , Bohao Zhao 1 , Tong Zhou 1 , Jiali Li 1 , Ming Liu 1 , Shuaishuai Hu 1 , Naisu Yang 1 , Yang Chen 1, 2 , Xinsheng Wu 1, 2
Background
Keratin-associated protein (KAP), the structural protein molecule of hair fibers, plays a key role in determining the physical properties of hair. Studies of Krtap11-1 have focused only on its localization. Functional studies of Krtap11-1 in hair follicle development have so far not been reported.
Objective
This study aimed to provide evidence for the role of Krtap11-1 in skin and hair development.
Methods
Full-length cloning and analysis of Krtap11-1 were conducted to ascertain its function. Overexpression vectors and interference sequences were constructed and transfected into RAB-9 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate the hair follicle developmental stage of Krtap11-1, the expression of different tissues, and the effects on other hair follicle development-related genes.
Results
The full length of cloned Krtap11-1 was 947 bp. Krtap11-1 was confirmed to be a hydrophilic protein localized mostly in mitochondria. The greatest mRNA expression was observed in skin. Using a follicle synchronization model, it was found that Krtap11-1 mRNA expression levels first increased then decreased over the passage of time, principally during hair follicle catagen and telogen. Following the overexpression of Krtap11-1, mRNA expression levels of the WNT-2, KRT17, BMP-2, and TGF-β-1 genes increased, and LEF-1 decreased (P < 0.05), the converse after the corresponding use of si-RNA interference.
Conclusions
Krtap11-1 exerts a promoting effect. The results provide novel insight into the relationship between hair follicle development and Krtap11-1 gene expression.
中文翻译:
Krtap11-1 在安哥拉兔 (Oryctolagus cuniculus) 毛囊发育过程中的特征和功能分析。
背景
角蛋白相关蛋白 (KAP) 是头发纤维的结构蛋白分子,在决定头发的物理特性方面起着关键作用。Krtap11-1 的研究只集中在它的定位上。迄今为止尚未报道 Krtap11-1 在毛囊发育中的功能研究。
客观的
本研究旨在为 Krtap11-1 在皮肤和头发发育中的作用提供证据。
方法
对 Krtap11-1 进行全长克隆和分析以确定其功能。构建过表达载体和干扰序列并转染到 RAB-9 细胞中。采用定量实时聚合酶链反应 (qRT-PCR) 研究 Krtap11-1 的毛囊发育阶段、不同组织的表达以及对其他毛囊发育相关基因的影响。
结果
克隆的 Krtap11-1 全长为 947 bp。Krtap11-1 被证实是一种亲水性蛋白质,主要位于线粒体中。在皮肤中观察到最大的 mRNA 表达。使用毛囊同步模型,发现 Krtap11-1 mRNA 表达水平随着时间的推移首先增加然后减少,主要是在毛囊退行期和休止期。Krtap11-1过表达后,WNT-2、KRT17、BMP-2和TGF-β-1基因的mRNA表达水平升高,LEF-1降低(P < 0.05),反之则相反。 si-RNA 干扰。
结论
Krtap11-1 发挥促进作用。结果为毛囊发育与 Krtap11-1 基因表达之间的关系提供了新的见解。