Schizophyllum commune is a wood-rotting filamentous fungus that secrets a homopolysaccharide called as schizophyllan. Schizophyllan has several applications such as enhanced oil recovery, pharmaceutical materials and an anti-cancer drug carrier. Biomass growth and schizophyllan production increase the viscosity of the cultivation medium, thus resulting in mass transfer limitation for the substrate. In this study, adding talc and aluminium oxide microparticles into the cultivation medium was studied to improve the fungal growth and morphology. The response surface methodology and one factor at a time were applied to find the effects of microparticles with different sizes and concentrations on the schizophyllan production. The optimum concentration and size of aluminium oxide microparticles were obtained as 20 g L⁻¹ and < 30 µm, respectively. Aluminium oxide microparticles in shake flask culture caused to increase the schizophyllan production from 10 to 15 g L⁻¹ and decrease the cultivation time from 10 to 7 days. The production yield also increased from 0.11 to 0.30 g of schizophyllan/g glucose. Bioreactor cultivation showed a twofold increase in schizophyllan production from 1.5 to 3 g L⁻¹. The results of this study suggested a significant increase in the production of schizophyllan using a low-cost “microparticle-enhanced cultivation” without any further optimization of the culture medium.

Schizophyllum community是一种腐烂木头的丝状真菌，它分泌一种称为裂叶多糖的同多糖。Schizophyllan 具有多种应用，例如提高石油采收率、药物材料和抗癌药物载体。生物质的生长和裂叶多糖的产生会增加培养基的粘度，从而导致基质的传质受限。在这项研究中，研究了在培养基中添加滑石粉和氧化铝微粒以改善真菌的生长和形态。应用响应面方法和一次一个因子来发现不同大小和浓度的微粒对裂殖多糖产量的影响。得到氧化铝微粒的最佳浓度和粒径为20 g L^-1和 < 30 µm，分别。摇瓶培养中的氧化铝微粒导致裂果聚糖产量从 10 g L ^-1增加到 15 g L ^-1并将培养时间从 10 天减少到 7 天。生产产量也从 0.11 克裂叶多糖/克葡萄糖增加到 0.30 克。生物反应器培养显示裂叶多糖产量从 1.5 到 3 g L ^-1增加了两倍。这项研究的结果表明，使用低成本的“微粒增强培养”可显着增加裂叶多糖的产量，而无需进一步优化培养基。