Methoxyfenozide is an insect growth disruptor belonging to the class of nonsteroidal ecdysone agonists. In the present study, methoxyfenozide (23% emulsifiable concentrate) is evaluated against newly molted fourth‐instar larvae of Culex pipiens L. and Culiseta longiareolata Macquart (Diptera: Culicidae), aiming to investigate its possible effects on growth and development. Larvae are exposed for 24 h under standard laboratory conditions in accordance with World Health Organization recommendations. The product is found to exhibit insecticidal activity against the two tested mosquito species with a concentration–response relationship. Moreover, based on the lethal concentrations determined, methoxyfenozide is slightly more toxic against C. pipiens than C. longiareolata. Furthermore, fourth‐instar larva of the two mosquito species are treated using two lethal concentrations (LC50 and LC90) of methoxyfenozide, and then individual survivals (larvae, pupae and adults) from larval treatment are subjected to a biometrical and biochemical study. The compound is found to interfere with growth by reducing the larval and pupal development duration. Moreover, the body volume and the main biochemical contents (proteins, carbohydrates and lipids) from different stages are affected. The overall results suggest that the ecdysone agonist under investigation interferes with the development process and has potential for mosquito control.

中文翻译：

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选择性杀虫剂（甲氧虫酰肼）对两种蚊子（Culex pipiens 和 Culiseta longiareolata）的活性：毒理学、生物统计学和生化研究

Methoxyfenozide 是一种昆虫生长干扰剂，属于非甾体蜕皮激素激动剂类。在本研究中，甲氧虫酰肼（23% 乳油）针对 Culex pipiens L. 和 Culiseta longiareolata Macquart（双翅目：Culicidae）新蜕皮的四龄幼虫进行评估，旨在研究其对生长和发育的可能影响。根据世界卫生组织的建议，幼虫在标准实验室条件下暴露 24 小时。发现该产品对两种测试蚊种表现出杀虫活性，并具有浓度-反应关系。此外，根据确定的致死浓度，甲氧虫酰肼对 C. pipiens 的毒性略高于 C. longiareolata。此外，使用两种致死浓度（LC50 和 LC90）的甲氧虫酰肼处理这两种蚊子的四龄幼虫，然后对幼虫处理后的个体存活（幼虫、蛹和成虫）进行生物统计学和生化研究。发现该化合物通过减少幼虫和蛹的发育持续时间来干扰生长。此外，不同阶段的身体体积和主要生化成分（蛋白质、碳水化合物和脂质）都会受到影响。总体结果表明，正在研究的蜕皮激素激动剂会干扰发育过程并具有控制蚊子的潜力。发现该化合物通过减少幼虫和蛹的发育持续时间来干扰生长。此外，不同阶段的身体体积和主要生化成分（蛋白质、碳水化合物和脂质）都会受到影响。总体结果表明，正在研究的蜕皮激素激动剂会干扰发育过程并具有控制蚊子的潜力。发现该化合物通过减少幼虫和蛹的发育持续时间来干扰生长。此外，不同阶段的身体体积和主要生化成分（蛋白质、碳水化合物和脂质）都会受到影响。总体结果表明，正在研究的蜕皮激素激动剂会干扰发育过程并具有控制蚊子的潜力。