The objective of this study was to determine the effects of extender osmolality on postthaw sperm quality and fertility in Sauger Sander canadensis. Fresh milt from 10 male Saugers was diluted by using base extenders with osmolalities of 350, 500, or 750 mOsm/kg (E350, E500, and E750, respectively) containing 10% dimethyl sulfoxide, frozen in LN₂ vapor, and stored. Sperm parameters (total motility, progressive motility, velocity, and viability) were assessed at different steps of the cryopreservation process (extended, equilibrated, and postthaw). Fertilization rates were compared between fresh and frozen sperm and at two sperm‐to‐egg ratios. All of the parameters that were measured, except for progressive motility, were reduced by cryopreservation. Extender 500 yielded the highest postthaw progressive motility (32.20 ± 3.86% [mean ± SD]) and velocity (84.97 ± 16.82 μm/s), whereas both E350 and E500 displayed the highest total motility (65.30 ± 4.24 and 68.70 ± 6.46%) and viability (80.60 ± 4.84 and 78.80 ± 3.91%), respectively. By contrast, E750 yielded the lowest postthaw velocity, viability, and total and progressive motility. Despite the increase in the motility parameters, fertilization in E350 (13.93%) was approximately double that in E500 (6.58%), although not statistically different. In conclusion, traditional isosmotic base extenders (E350) were found to be superior to hypertonic base extenders in the preservation of Sauger milt. These results serve as a starting point for future investigations of cryopreservation potential for Sauger spermatozoa that work toward developing a freezing protocol that is more suitable for large‐scale application.

中文翻译：

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使用高渗介质冷冻贮藏索伯精子

本研究的目的是确定补充渗透压对索格桑德（Can Sauger canadensis）解冻后精子质量和育性的影响。通过使用渗透压为350、500或750 mOsm / kg（分别为E350，E500和E750）的含10％二甲亚砜的基本增量剂稀释10个雄性Sauger的新鲜鱼鳞，并在LN _2中冷冻蒸气，并储存。在冷冻保存过程的不同步骤（延长，平衡和融化后）评估精子参数（总运动性，进行性运动，速度和生存力）。比较了新鲜精子和冷冻精子的受精率，以及两个精子与卵的比率。除进行性运动外，所有测定的参数均通过冷冻保存降低。Extender 500产生最高的解冻后进行性运动（32.20±3.86％[平均值±SD]）和速度（84.97±16.82μm/ s），而E350和E500均显示出最高的总运动性（65.30±4.24和68.70±6.46％）和生存力（分别为80.60±4.84和78.80±3.91％）。相比之下，E750产生的解冻后速度，活力以及总运动和进行性运动最低。尽管运动参数有所增加，尽管没有统计学差异，但E350的受精率（13.93％）约为E500的两倍（6.58％）。总之，发现传统的等渗碱补充剂（E350）在保存索格麦芽的过程中优于高渗碱补充剂。这些结果为将来研究萨格（Sauger）精子的冷冻保存潜力提供了一个起点，该研究旨在开发更适合大规模应用的冷冻方案。