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New data about Australophlebotomus notteghemae Léger & Pesson, 1993 (Diptera, Psychodidae)
New Zealand Entomologist ( IF 0.3 ) Pub Date : 2019-01-02 , DOI: 10.1080/00779962.2019.1644774 Jérôme Depaquit 1 , Laurianne Mamatui 1 , Denis Augot 1 , Matthieu L. Kaltenbach 1
New Zealand Entomologist ( IF 0.3 ) Pub Date : 2019-01-02 , DOI: 10.1080/00779962.2019.1644774 Jérôme Depaquit 1 , Laurianne Mamatui 1 , Denis Augot 1 , Matthieu L. Kaltenbach 1
Affiliation
ABSTRACT Two endemic Australophlebotomus species have been recorded in New Caledonia: Au. notteghemae Léger & Pesson and Au. maduloae Léger & Pesson, as reported in 1993. At that time, the authors mainly based their description on male holotypes, and tentatively associated two different females called ‘A’ and ‘B’ to each male. Very scarce in New Caledonia, these two species have been reported on very few occasions, with specimens coming from various locations of this island. Having now access to two Australophlebotomus females and two Au. notteghemae males caught in 1997 in the Touaourou cave (New Caledonia), our aim was to validate the male-female association in Au. notteghemae and to add to the description of this species. One pair was subjected to a DNA analysis while the other pair was used for morphological studies. Cytochrome b of the mtDNA, and the D1, D2 and D8 domains of the 28S rDNA were sequenced. For each marker, full sequence homology (100%, no mutation) was observed for the processed male and female. Original drawings and new morphological data about Au. notteghemae are provided. Both molecular and morphological evidences strongly associate female ‘A’ to the male Au. notteghemae specimen.
中文翻译:
Australophlebotomus notteghemae Léger & Pesson, 1993 (Diptera, Psychodidae) 的新数据
摘要 在新喀里多尼亚记录了两种地方性 Austraphlebotomus 物种:Au。notteghemae Léger & Pesson 和 Au。maduloae Léger & Pesson,如 1993 年报道的那样。当时,作者主要基于雄性正模的描述,并暂时将两个不同的雌性称为“A”和“B”。在新喀里多尼亚非常稀少,这两种物种的报道很少,标本来自该岛的不同地点。现在可以接触到两个 Australophlebotomus 雌性和两个 Au。1997 年在 Touaourou 洞穴(新喀里多尼亚)捕获的 notteghemae 雄性,我们的目的是验证 Au 的男女关联。notteghemae 并添加到该物种的描述中。一对进行 DNA 分析,而另一对用于形态学研究。对 mtDNA 的细胞色素 b 和 28S rDNA 的 D1、D2 和 D8 域进行测序。对于每个标记,处理后的男性和女性均观察到全序列同源性(100%,无突变)。关于 Au 的原始图纸和新的形态数据。提供了nottegemae。分子和形态学证据都将雌性“A”与雄性 Au 密切相关。notteghae 标本。
更新日期:2019-01-02
中文翻译:
Australophlebotomus notteghemae Léger & Pesson, 1993 (Diptera, Psychodidae) 的新数据
摘要 在新喀里多尼亚记录了两种地方性 Austraphlebotomus 物种:Au。notteghemae Léger & Pesson 和 Au。maduloae Léger & Pesson,如 1993 年报道的那样。当时,作者主要基于雄性正模的描述,并暂时将两个不同的雌性称为“A”和“B”。在新喀里多尼亚非常稀少,这两种物种的报道很少,标本来自该岛的不同地点。现在可以接触到两个 Australophlebotomus 雌性和两个 Au。1997 年在 Touaourou 洞穴(新喀里多尼亚)捕获的 notteghemae 雄性,我们的目的是验证 Au 的男女关联。notteghemae 并添加到该物种的描述中。一对进行 DNA 分析,而另一对用于形态学研究。对 mtDNA 的细胞色素 b 和 28S rDNA 的 D1、D2 和 D8 域进行测序。对于每个标记,处理后的男性和女性均观察到全序列同源性(100%,无突变)。关于 Au 的原始图纸和新的形态数据。提供了nottegemae。分子和形态学证据都将雌性“A”与雄性 Au 密切相关。notteghae 标本。