当前位置：
X-MOL 学术
›
Mar. Freshw. Behav. Physiol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Immunohistochemical localization of a H2S-generating enzyme and its potential molecular target in hepatopancreas of the freshwater crayfish Procambarus clarkii
Marine and Freshwater Behaviour and Physiology ( IF 1 ) Pub Date : 2019-05-04 , DOI: 10.1080/10236244.2019.1659103 Keqiang Wei 1 , Yue Wei 2 , Changxia Song 1
Marine and Freshwater Behaviour and Physiology ( IF 1 ) Pub Date : 2019-05-04 , DOI: 10.1080/10236244.2019.1659103 Keqiang Wei 1 , Yue Wei 2 , Changxia Song 1
Affiliation
ABSTRACT There is increasing evidence showing that the gasotransmitter hydrogen sulfide (H2S) and NF-κB-mediated signaling may play an important role in regulating immune response. However, essentially nothing is known about them in crustaceans, especially the red swamp crayfish (Procambarus clarkii). In this work, we used immunohistochemical method to investigate, for the first time, the presence and distribution of a H2S biosynthetic enzyme (cystathionine β-synthase, CBS) and a member of NF-κB family (RelA/p65) in crayfish hepatopancreas. Results indicated that (1) CBS-like and NF-κB p65-like immunopositive cells coexisted in hepatopancreas; (2) NF-κB p65 was diffusely localized and highly expressed in the cytoplasm of R- and B-cells, but CBS was observed mainly in connective tissue, especially in sinusoids; (3) and normally, at mean optical density (MOD) level, their expression was about 0.0032 ± 0.0028 and 0.0072 ± 0.0039, and proportion (%) of positive cells was 14.37 ± 5.76 and 23.5 ± 14.3, respectively.
更新日期：2019-05-04
京公网安备 11010802027423号