In this study, a novel HSP90 gene (PdHSP90) was cloned from Prorocentrum donghaiense. The full length of PdHSP90 is 2443 bp, with 5′-UTR of 111 bp, 3′-UTR of 199 bp, and open reading frame (ORF) of 2133 bp. The ORF encodes a polypeptide of 710 amino acid residues, with molecular weight of approximately 85.81 kDa and isoelectric point of 4.95. Phylogenetic analysis revealed that PdHSP90 belongs to the dinoflagellate clade and is closely related to HSP90s from P. minimum and P. micans. PdHSP90 expression increased under the thermal stress (p < 0.05), with the highest changes of 7.6-fold at 10°C 2 h post treatment compared to the control. PdHSP90 expression was significantly upregulated (1- to 10.8-fold change) (p < 0.05) with increasing concentrations of copper from 0 µM to 31.25 µM. PdHSP90 expression level under 176 µM nitrogen was nearly equal to that under 88 µM nitrogen, which were 4.5- and 4.6-fold higher, respectively, compared with the control (p < 0.05). Upon treatment with phosphorus limitation from 36 µM to 3.6 µM, PdHSP90 transcription level increased 11.4-fold (p < 0.05) and then decreased. These results provide new insights into the molecular mechanism underlying adaptation of P. donghaiense and other harmful dinoflagellates in response to diverse environmental factors.

在这项研究中，从东海原螯虾克隆了一个新的HSP90基因（PdHSP90）。PdHSP90的全长为2443 bp，5'-UTR为111 bp，3'-UTR为199 bp，开放阅读框（ORF）为2133 bp。ORF编码一个710个氨基酸残基的多肽，分子量约为85.81 kDa，等电点为4.95。系统发育分析表明，PdHSP90属于甲藻和进化密切相关，从HSP90s最低P.和海洋原甲藻。在热胁迫下PdHSP90表达增加（p <0.05），与对照相比，在治疗后2小时10°C时变化最大为7.6倍。 随着铜浓度从0 µM增加到31.25 µM，PdHSP90表达显着上调（变化1到10.8倍）（p <0.05）。在176 µM氮气下，PdHSP90表达水平几乎与在88 µM氮气下的表达水平相等，分别比对照高4.5倍和4.6倍（p  <0.05）。在磷限度从36 µM到3.6 µM处理后，PdHSP90转录水平增加了11.4倍（p  <0.05），然后下降。这些结果为了解P适应的分子机制提供了新见解。。donghaiense和其他有害的鞭毛藻对多种环境因素的反应。