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Monitoring the genetic effects of broodstock enhancement of silver carp (Hypophthalmichthys molitrix) in middle Yangtze River based on cytb gene and D-loop sequences
Journal of Freshwater Ecology ( IF 1.3 ) Pub Date : 2019-01-01 , DOI: 10.1080/02705060.2019.1588795
Huijuan Chen 1, 2 , Dengqiang Wang 2 , Jie Guo 2, 3 , Xinbin Duan 2 , Shaoping Liu 2 , Daqing Chen 1, 2 , Yun Li 1
Affiliation  

Abstract In this study, we first evaluated the genetic effects of the stock enhancement program of silver carp (Hypophthalmichthys molitrix) on wild populations in the middle Yangtze River using the sequences of the mitochondrial Cytochrome b gene and control region (D-loop). 129 cytb individuals and 135 D-loop individuals were sequenced successfully, and the results obtained by two markers were similar. T test results showed that there was no significant difference in haplotype diversity (Hd) values and nucleotide diversity (Pi) values before and after release. The exact test result of population differentiation based on haplotype frequencies was consistent with no significant change in haplotype frequency after release. Pairwise genetic differentiation index (FST) and gene flow (Nm) revealed no genetic differentiation among four populations. Results of the Bayesian clustering analysis performed with STRUCTURE and analysis of molecular variance (AMOVA) confirmed that the genetic component did not change after release. In summary, there was no significant genetic effect of broodstock enhancement of silver carp on wild populations in the middle Yangtze River.

中文翻译:

基于cytb基因和D-loop序列的长江中游鲢(Hypophthalmichthys molitrix)亲体增殖遗传效应监测

摘要 在本研究中,我们首先利用线粒体细胞色素 b 基因和控制区(D-loop)的序列评估了鲢鱼(Hypophthalmichthys molitrix)种群增殖计划对长江中游野生种群的遗传效应。成功测序了129个cytb个体和135个D-loop个体,两个标记得到的结果相似。T检验结果表明,释放前后单倍型多样性(Hd)值和核苷酸多样性(Pi)值无显着差异。基于单倍型频率的群体分化的确切测试结果与释放后单倍型频率无显着变化一致。成对遗传分化指数(FST)和基因流(Nm)显示四个种群之间没有遗传分化。使用 STRUCTURE 和分子方差分析 (AMOVA) 进行的贝叶斯聚类分析的结果证实,遗传成分在释放后没有发生变化。综上所述,鲢鱼亲体增强对长江中游野生种群的遗传效应不显着。
更新日期:2019-01-01
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