Two specific characteristics of stem cells, self-renewal and their differentiation capacity have made them important for vast scientific areas, particularly in the molecular and cell biology of stem cells, as well as regenerative medicine. Gene targeting plays a pivotal role in creating modified stem cells, which are considered the mainstay in regenerative medicine. The phiC31 integrase system, as a site-specific recombinase, could be a straightforward and efficient approach for this aim. According to the high potential of the application of phiC31 integrase system in stem cells, the present paper reviews previous studies, placing the focus on stem cell types and their application.

干细胞的两个特殊特性，即自我更新及其分化能力，使其在广阔的科学领域中发挥着重要作用，特别是在干细胞的分子和细胞生物学以及再生医学领域。基因靶向在创建修饰的干细胞中起着关键作用，而干细胞被认为是再生医学的支柱。phiC31整合酶系统作为一种位点特异性重组酶，可能是实现此目标的一种简单有效的方法。鉴于phiC31整合酶系统在干细胞中的巨大应用潜力，本文综述了以前的研究，重点是干细胞类型及其应用。