This study investigated the antidiabetic and antioxidant activities of the extracts from three parts of Alstonia boonei. Sequential extraction of leaves, stem and root bark samples was done with the solvents of increasing polarity and these extracts were assayed for total phenolic content and in vitro antioxidant and antidiabetic activities using different experimental models. The electron donating, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide radical scavenging activities of all the extracts were investigated in this study. The ability of these extracts to inhibit α-glucosidase and α-amylase was also examined in vitro. The ethanol extract of leaves showed the highest total phenolic content compared to other extracts. All extracts showed free radical scavenging abilities in different experimental models. The ethanol and aqueous extracts significantly (p < 0.05) inhibited the activities of key enzymes linked to type 2 diabetes. GC-MS analysis of the extracts from different plant parts revealed the presence of sterols, aromatics, aliphatic acids and esters. These compounds showed a high binding affinity with α-glucosidase and α-amylase enzymes under molecular docking studies. Data of this study suggest that the different parts of A. boonei, especially the ethanol and aqueous extracts of leaves, possess potent antioxidant and diabetes related enzymes inhibitory activities that could be further exploited in the development of novel antidiabetic medicines.

本研究考察了三香参Alstonia boonei提取物的抗糖尿病和抗氧化活性。用极性增加的溶剂对叶，茎和根皮样品进行顺序提取，并使用不同的实验模型对这些提取物的总酚含量以及体外抗氧化和抗糖尿病活性进行了测定。本研究研究了所有提取物的给电子，1,1-二苯基-2-甲基苯并肼（DPPH）和一氧化氮自由基清除活性。还在体外检查了这些提取物抑制α-葡萄糖苷酶和α-淀粉酶的能力。。与其他提取物相比，叶子的乙醇提取物显示出最高的总酚含量。在不同的实验模型中，所有提取物均具有清除自由基的能力。乙醇和水提取物显着（p <0.05）抑制了与2型糖尿病有关的关键酶的活性。对来自不同植物部位的提取物进行的GC-MS分析表明，存在固醇，芳香族化合物，脂族酸和酯。在分子对接研究中，这些化合物与α-葡萄糖苷酶和α-淀粉酶具有很高的结合亲和力。这项研究的数据表明，A。boonei的不同部分尤其是叶子的乙醇和水提取物，具有强大的抗氧化剂和糖尿病相关酶抑制活性，可以在开发新型抗糖尿病药物中进一步利用。