Abstract In the aluminum and steel industry there is no specialized bioremediation of the pure or spent hydrocarbon rolling oils. Hazardous waste management companies remove the spent oils which eventually end up being dumped at local hazardous material dump sites. The lack of an environmentally friendly strategy to reduce the hazardous waste from factories was incentive for this research. The aim was to determine and monitor an indigenous Achromobacter aegrifaciens bacterial biotransformation/biodegradation of Kerosene based rolling oils including the additives: methyl laurate, lauric acid and Shellsol D100. This was done via dehydrogenase assays and Gas Chromatography-Mass Spectrometry. Achromobacter aegrifaciens bacterial biotransformation was also compared to that of an exogenous consortium of Lysinibacillus fusiformis and Pseudomonas synaxantha. Results were analyzed statistically, using SPSS version 24. The mean differences were significant at the 0.05 level for both DHA and GCMS. Further, subterminal oxidation was determined to be the main activation mechanism for the biotransformation. Methylation occurred during rolling oil biotransformation but was more prevalent in biotransformations of the additives, especially methyl laurate. Methylation resulted in less efficient biotransformations compared to subterminal oxidation. This bioremediation strategy can now be further established for larger volume bioreactors as we established that the toxic monoaromatic compounds were successfully degraded at the end of five weeks making this bioremediation strategy a viable alternative to the current dumping by waste companies.

中文翻译：

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气相色谱-质谱法测定细菌胞内和胞外生物转化化合物及煤油基工业轧制油的生物降解

摘要 在铝和钢铁工业中，没有对纯烃轧制油或废烃轧制油进行专门的生物修复。危险废物管理公司清除废油，这些废油最终被倾倒在当地的危险材料倾倒场。缺乏减少工厂有害废物的环保战略是这项研究的动力。目的是确定和监测基于煤油的轧制油的原生无色杆菌细菌生物转化/生物降解，包括添加剂：月桂酸甲酯、月桂酸和 Shellsol D100。这是通过脱氢酶测定和气相色谱-质谱法完成的。Achromobacter aegrifaciens 细菌生物转化也与梭形赖氨酸杆菌和假单胞菌的外源聚生体进行了比较。使用 SPSS 24 版对结果进行统计学分析。 DHA 和 GCMS 的平均差异在 0.05 水平上显着。此外，亚末端氧化被确定为生物转化的主要激活机制。甲基化发生在轧制油生物转化过程中，但在添加剂的生物转化中更为普遍，尤其是月桂酸甲酯。与亚末端氧化相比，甲基化导致生物转化效率较低。