Abstract Arsenic (As), a major groundwater contaminant has gained attention as a surface water pollutant, due to its deposition on agricultural fields and nearby waterbodies as a result of over usage of groundwater. Several plants have the unique ability to phyto-remediate and accumulate As but differ in their potential. The present study focuses on removal of arsenic by Pistia stratiotes exposed to 10, 50, 100, and 200 ppb of As (As2O3), and simultaneous anti-oxidative changes in plants facilitating arsenic tolerance in these aquatic macrophytes. The removal percentage rate of As was found to decrease with the increase in initial As levels in the hydroponic solution over an experimental period of 28 days. The maximum removal percentage was obtained at the 10 ppb As level (61.42%). Changes in concentration of anti-oxidative enzymes, i.e., catalase, ascorbate peroxidase and guaiacol peroxidase, were measured over intervals of 7 days. Significant changes were observed in activities of catalase, guaiacol peroxidase, and ascorbate peroxidase with highest catalase activity on 28th day at 200 ppb As concentration. Ascorbate peroxidase activity varied over 28-day period with increased activity from seventh day. Activity of guaiacol peroxidase showed the same trend as ascorbate peroxidase. The present research work highlights the assessment of anti-oxidative enzymatic changes in Pistia stratiotes due to As exposure and uptake.

中文翻译：

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Pistia striotes 对砷的植物治疗解毒及其抗氧化酶促变化的评估

摘要 砷（As）是一种主要的地下水污染物，由于过度使用地下水而沉积在农田和附近的水体中，作为一种地表水污染物而受到关注。一些植物具有独特的植物修复和积累砷的能力，但它们的潜力不同。本研究的重点是通过暴露于 10、50、100 和 200 ppb 砷 (As2O3) 的 Pistia 层状藻去除砷，以及同时促进这些水生大型植物的砷耐受性的植物抗氧化变化。在 28 天的实验期间，发现 As 的去除百分比随着水培溶液中初始 As 水平的增加而降低。在 10 ppb 砷水平下获得最大去除百分比 (61.42%)。抗氧化酶，即过氧化氢酶的浓度变化，抗坏血酸过氧化物酶和愈创木酚过氧化物酶每隔 7 天测量一次。过氧化氢酶、愈创木酚过氧化物酶和抗坏血酸过氧化物酶的活性在第 28 天在 200 ppb 砷浓度下观察到显着变化，其中过氧化氢酶活性最高。抗坏血酸过氧化物酶活性在 28 天期间随第 7 天起活性增加而变化。愈创木酚过氧化物酶的活性表现出与抗坏血酸过氧化物酶相同的趋势。目前的研究工作重点评估了由于暴露和摄取砷而导致的 Pistia 层状体中抗氧化酶的变化。抗坏血酸过氧化物酶活性在 28 天期间随第 7 天起活性增加而变化。愈创木酚过氧化物酶的活性表现出与抗坏血酸过氧化物酶相同的趋势。目前的研究工作重点评估了由于暴露和摄取砷而导致的 Pistia 层状体中抗氧化酶的变化。抗坏血酸过氧化物酶活性在 28 天期间随第 7 天起活性增加而变化。愈创木酚过氧化物酶的活性表现出与抗坏血酸过氧化物酶相同的趋势。目前的研究工作重点评估了由于暴露和摄取砷而导致的 Pistia 层状体中抗氧化酶的变化。