RNA editing changes the nucleotides at the transcript level of mitochondrial genes which results in synthesis of functional proteins. This study was designed to find the editing sites which could be implicated in male fertility restoration and to develop editing based markers for differentiation of cytoplasmic male sterility and maintainer lines from each other. DNA and RNA from young panicles were isolated from three-line system of hybrid rice PRH10, wild abortive (WA) cytoplasm based male sterile (A line Pusa 6A), maintainer (B line Pusa 6B) and restorer (R line PRR78) lines. Pusa 6A and PRR78 having the same WA cytoplasm are allo-nuclear and iso-cytpolasmic lines. The genomic and cDNA amplicons for eight mitochondrial genes (18SrRNA, atp6, atp9, cobII, coxI, coxIII, nadI and rps3) were sequenced and compared. Differences in genomic and cDNA sequences were considered as editing. Two hundred and thirty editing sites having base substitution or insertion/deletion were identified with the highest in 18SrRNA (5.74%) and the lowest in coxI (0.60%). The highest editing sites were observed in fertile maintainer Pusa 6B followed by PRR78 and Pusa 6A, of which random five editing sites in five different rice mitochondrial transcripts namely atp9, cobII, coxIII, rps3 and 18SrRNA were chosen and validated through cleaved amplified polymorphism sequence (CAPS) analysis and found to be partially edited in four genes. The identical editing sites of different mitochondrial genes from maintainer and restorer lines might reflect their possible contribution to fertility restoration of sterile WA cytoplasm.

RNA编辑可改变线粒体基因转录本上的核苷酸，从而合成功能蛋白。本研究旨在发现可能与雄性生殖力恢复有关的编辑位点，并开发出基于编辑的标记，以区分胞质雄性不育和维持系。从杂交水稻PRH10，野生流产（WA）细胞质雄性不育（A系Pusa 6A），维持系（B系Pusa 6B）和恢复系（R系PRR78）的三系系统中分离出幼穗的DNA和RNA。具有相同WA细胞质的Pusa 6A和PRR78是同核细胞系和同胞胞体系。八个线粒体基因（18SrRNA，atp6，atp9，对cobII，coxI，coxIII，nadI和rps3进行测序和比较。基因组和cDNA序列的差异被视为编辑。鉴定出具有碱基取代或插入/缺失的230个编辑位点，其中18SrRNA最高（5.74％），而coxI最低（0.60％）。在可育维持系Pusa 6B，PRR78和Pusa 6A中观察到最高的编辑位点，其中在五个不同的水稻线粒体转录物atp9，cobII，coxIII，rps3和18SrRNA中随机出现五个编辑位点选择并通过切割的扩增多态性序列（CAPS）分析进行验证，发现其在四个基因中被部分编辑。来自维持系和恢复系的不同线粒体基因的相同编辑位点可能反映了它们对无菌WA细胞质的繁殖力恢复的可能贡献。