Genetic transformation of rice (Oryza sativa L.) by introducing beneficial traits is now a central research instrument in plant physiology and a practical tool for plant improvement. Many approaches are verified for stable introduction of foreign genes into the plant genome. The review examined the different constraints that limit the success of rice genetic transformation via Agrobacterium-mediated approach and suggested possible solutions. Explant identification, gene transfer technique and construct to tailor the integration, transgene expression without collateral to genetic damage and transformant selection are among the technical challenges affecting the rice transformation. Despite the contests, Agrobacterium- mediated transformation system has been a better option for producing transgenic rice varieties because of its exact T-DNA processing and simple integration of low copy-number transgene. This information is necessary for improving the transformation system for recalcitrant rice varieties.

通过引入有益的性状对水稻（Oryza sativa L.）进行遗传转化，现已成为植物生理学的主要研究手段，也是植物改良的实用工具。验证了许多将外源基因稳定导入植物基因组的方法。审查审查了限制通过农杆菌介导的方法成功进行水稻遗传转化的各种限制因素，并提出了可能的解决方案。影响水稻转化的技术挑战包括外植体鉴定，基因转移技术和适应整合的构建，无遗传损害和转基因选择的转基因表达。尽管有比赛，农杆菌-介导的转化系统由于其精确的T-DNA处理和低拷贝数转基因的简单整合，已成为生产转基因水稻品种的更好选择。该信息对于改进顽calc水稻品种的转化系统是必要的。