Osmoregulatory mechanisms have been investigated in different groups of bacteria. Nevertheless, the molecular processes of deep-sea microorganism in response to salt stress have not been investigated. In this study, the temporal gene expression patterns of the deep-sea bacterium Shewanella piezotolerans WP3 in response to high salinity stress were evaluated through whole-genome microarray analysis. A total of 35, 13, and 19 genes were differentially expressed after WP3 was exposed to high salinity stress for 30, 60, and 90 min, respectively. Only five genes were identified as commonly differentially expressed. The hierarchical clustering analysis of the gene expression patterns revealed that WP3 uses common adaptive strategies to cope with the multiple environmental stresses. The results of this study provide the first glimpse into the changes in global gene expression in benthic bacterium under high salt stress.

已经在不同种类的细菌中研究了渗透调节机制。然而，尚未研究深海微生物响应盐胁迫的分子过程。在这项研究中，深海细菌Shewanella piezotolerans的时间基因表达模式通过全基因组微阵列分析评估了对高盐分胁迫响应的WP3。在WP3分别暴露于高盐度胁迫30、60和90分钟后，总共35、13和19个基因差异表达。只有五个基因被鉴定为通常差异表达。基因表达模式的层次聚类分析表明，WP3使用常见的适应策略来应对多种环境压力。该研究结果首次揭示了高盐胁迫下底栖细菌整体基因表达的变化。