Calotropis gigantea (L.) W. T. Aiton and Centella asiatica (L.) Urban have been medicinally used throughout Bangladesh. In this study, karyotypic features of these two species were studied with orcein staining and banding with two base specific fluorochromes such as chromomycine A₃ (CMA) and 4´, 6 diamidino-2-phenylindole (DAPI) for authentic cytogenetical characterization. 2n = 22 and 2n = 18 somatic chromosomes were found in C. gigantea and C. asiatica, respectively. The total chromosome length of C. gigantea and C. asiatica were 45.35 ± 2.64 μm and 63.63 ± 3.19 μm, respectively. The total form percent (TF%), karyotype asymmetry index (AsK%) and karyotype symmetry index (Syi index) were 47.61, 52.39 and 90.87 in C. gigantea and 39.58, 60.42 and 65.51 in C. asiatica, respectively. Both species were found to possess 1A karyotype. In C. gigantea, 2 CMA- and 4 DAPI-bands were observed whereas C. asiatica had 16 CMA- and 6 DAPI-bands. Therefore, the compiled data generated from chromosome analysis on orcein, CMA- and DAPI-banding will be useful for cytogenetical characterization of these two species.

Calotropis gigantea（L.）WT Aiton和Centella asiatica（L.）Urban已在整个孟加拉国被药用。在这项研究中，对这两个物种的核型特征进行了海藻素染色研究，并用两种碱基特异性荧光染料（如铬霉素A ₃（CMA）和4´，6 diamidino-2-phenylindole（DAPI））进行了条带化​​，以进行真实的细胞遗传学表征。在巨型梭状芽胞杆菌和亚洲梭状芽胞杆菌中分别发现2n = 22和2n = 18体细胞染色体。C. gigantea和C.asiatica的总染色体长度分别为45.35±2.64μm和63.63±3.19μm。总百分比形式（TF％），核型不对称指数（ASK％）和核型对称性指数（SYI指数）分别为47.61，52.39和90.87中C.巨和39.58，60.42和65.51中积雪草，分别。发现这两个物种都具有1A核型。在C.巨，2 CMA-和4 DAPI带中观察到，而积雪草得到16 CMA-和6 DAPI带。因此，从原始蛋白，CMA和DAPI条带的染色体分析生成的编译数据将对这两个物种的细胞遗传学表征很有用。