Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C. J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C. B. Müller, E. Jörg, “Image scanning microscopy, “Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, “Superresolution by image scanning microscopy using pixel reassignment,” Opt. Lett. 38(15) 2889–2892 (2013)]. Here, we use a tailor-made optical fiber and 19 avalanche photodiodes (APDs) as parallel detectors to upgrade our existing confocal microscopy, termed as parallel-detection super-resolution (PDSR) microscopy. In order to obtain the correct shift value, we use the normalized 2D cross correlation to calculate the shifting value of each image. We characterized our system performance by imaging fluorescence beads and applied this system to observing the 3D structure of biological specimen.

中文翻译：

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基于平行检测的超分辨率显微镜

基于像素重新分配的图像扫描显微镜可以提高共焦分辨率限制，而不会大大降低图像信噪比 (SNR) [CJR Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988) ）。CB Müller, E. Jörg，“图像扫描显微镜，”物理。牧师莱特。104（19）198101（2010）。CJR Sheppard、SB Mehta、R. Heintzmann，“使用像素重新分配的图像扫描显微镜的超分辨率”，选项。莱特。38（15）2889–2892（2013）]。在这里，我们使用定制的光纤和 19 个雪崩光电二极管 (APD) 作为并行检测器来升级我们现有的共聚焦显微镜，称为并行检测超分辨率 (PDSR) 显微镜。为了得到正确的移位值，我们使用归一化的二维互相关来计算每幅图像的移位值。