To date, numerous studies have been performed to elucidate the complex cellular dynamics in skin diseases, but few have attempted to characterize these cellular events under conditions similar to the native environment. To address this challenge, a three-dimensional (3D) multimodal analysis platform was developed for characterizing in vivo cellular dynamics in skin, which was then utilized to process in vivo wound healing data to demonstrate its applicability. Special attention is focused on in vivo biological parameters that are difficult to study with ex vivo analysis, including 3D cell tracking and techniques to connect biological information obtained from different imaging modalities. These results here open new possibilities for evaluating 3D cellular dynamics in vivo, and can potentially provide new tools for characterizing the skin microenvironment and pathologies in the future.

中文翻译：

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受伤皮肤细胞动力学的视频速率多模态多光子成像和三维表征

迄今为止，已经进行了大量研究来阐明皮肤疾病中复杂的细胞动力学，但很少有人试图在类似于自然环境的条件下表征这些细胞事件。为了应对这一挑战，开发了三维（3D）多模态分析平台，用于表征皮肤体内细胞动力学，然后用于处理体内伤口愈合数据以证明其适用性。特别关注的是难以通过离体分析进行研究的体内生物参数，包括 3D 细胞追踪和连接从不同成像方式获得的生物信息的技术。这些结果为评估体内 3D 细胞动力学开辟了新的可能性，并有可能为未来表征皮肤微环境和病理学提供新工具。