Electricigens can transfer electrons that produced in intracellular metabolic processes to cellular surface to restore extracellular insoluble electron acceptors (extracellular electron transfer, EET). To uncover the molecular mechanisms underlying EET processes, we integrated transcriptome changes accompanying such processes with molecular network. Firstly, time-series expression datasets for Shewanella oneidensis MR-1 under limited/changed [Formula: see text] conditions were obtained from the GEO database, and a total of 336 common differentially expressed genes (DEGs) were identified. Then, we constructed the protein–protein interaction (PPI) network that involved in EET processes from these DEGs. Furthermore, by using centralization analysis and community detection, network analysis of the PPI network was performed. Although the fundamental EET genes are similar to previous studies, important new genes have been discovered. Taking together, our study identified many literature-validated genes critical to EET processes, and also proposed some novel genes that were putatively involved in EET processes.

中文翻译：

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常见差异基因的网络分析确定了细胞外电子转移过程中的关键基因和重要模块

电激元可以将细胞内代谢过程中产生的电子转移到细胞表面，以恢复细胞外不溶性电子受体（细胞外电子转移，EET）。为了揭示 EET 过程的分子机制，我们将伴随这些过程的转录组变化与分子网络相结合。首先，从GEO数据库中获得了Shewanella oneidensis MR-1在有限/变化[公式：见正文]条件下的时间序列表达数据集，共鉴定出336个常见差异表达基因（DEGs）。然后，我们从这些 DEG 构建了参与 EET 过程的蛋白质-蛋白质相互作用 (PPI) 网络。此外，通过使用集中分析和社区检测，对 PPI 网络进行了网络分析。虽然基本的 EET 基因与以前的研究相似，但已经发现了重要的新基因。总之，我们的研究确定了许多经文献验证的对 EET 过程至关重要的基因，并提出了一些推定参与 EET 过程的新基因。