Our aim is to study the roles of a new emerging group of non-coding RNAs, circRNAs, in tomato (Solanum lycopersicum L.) plants grown at the combination of drought and heat, two of the most usual stress conditions known to frequently happen in field. Tomato seedlings from cultivar ‘Jinling Meiyu’ were treated without stresses (control), at water shortage, high temperature and subjected the multiple stresses. In total, 467 circRNAs were identified with 87.82% from exon using high throughput sequencing technology. Among the circRNAs, 70 were from chr1 with the range from 23 to 49 from the other chromosomes. In detail, 156 circRNAs were shared in the four libraries, while 21, 17 and 36 circRNAs were only shown in drought, heat and multiple stresses libraries, respectively. Through a differential expression analysis, four, seven and nine circRNAs were differentially regulated in tomato at drought, heat and multiple stresses as compared with control. These circRNAs played roles on photosynthesis, starch and sucrose metabolism, RNA transport, RNA degradation, spliceosome, ribosome, etc. Our study underlined the potential role of circRNAs involved in the abiotic stress response in tomato, which might pave the way for studying biological roles of circRNAs responding to multiple stresses in plants.

我们的目的是研究一组新兴的非编码RNAs circRNA在番茄中的作用（茄属植物lycopersicumL.）在干旱和高温的共同作用下生长的植物，这是已知在田间经常发生的两种最常见的胁迫条件。在缺水，高温下，对'金陵梅雨'品种的番茄幼苗进行了无胁迫处理（对照），并承受了多种胁迫。使用高通量测序技术，总共从外显子中鉴定出467种circRNA，占87.82％。在circRNA中，有70个来自chr1，其他染色体的范围为23至49。详细地，在四个文库中共有156个circRNA，而分别仅在干旱，高温和多重胁迫文库中显示了21、17和36个circRNA。通过差异表达分析，与对照相比，在干旱，高温和多重胁迫下，番茄中有四个，七个和九个circRNA受到差异调节。