To improve the understanding of molecular mechanisms of anther and/or pollen development in Chili pepper, in the present study, full-length cDNA and DNA sequences of the pollen development-related gene CaMF5 were obtained from the anthers of a Capsicum annuum nuclear male-fertile line. Sequence analysis indicated that the full length of CaMF5 was 747 bp, containing a maximum opening reading frame of 447 bp. Amino acid sequence alignment and phylogenetic analysis revealed that CaMF5 shared approximately 37%–77% homology with a series of uncharacterized or hypothetical proteins and late embryogenesis abundant (LEA) proteins from other plants. However, no LEA structural domain was detected in CaMF5, which indicated that it might be a new type of LEA gene. CaMF5 was only expressed in flower buds at stages 7 and 8 and in open flowers of the male-fertile line, whereas it exhibited no expression in any examined organs of the male-sterile line. In addition, CaMF5 showed the highest transcript abundance in the anthers of the male-fertile line, with no expression being detected in any other examined organs, such as the sepals, petals, pistils, roots, stems, or leaves. Taken together, our results suggest that CaMF5 is an anther-specific gene that might encode a new type of LEA protein related to anther and/or pollen development in C. annuum.

为了提高在辣椒花药和/或花粉发育的分子机制的理解，在本研究中，花粉的发育相关的基因的全长cDNA和DNA序列CaMF5从一个的花药获得辣椒核男性为肥沃的线。序列分析表明，CaMF5的全长为747 bp，最大阅读框为447 bp。氨基酸序列比对和系统发育分析表明，CaMF5与一系列未表征或假设的蛋白质以及来自其他植物的晚期胚胎发生丰富（LEA）蛋白质具有大约37％–77％的同源性。但是，在CaMF5中未检测到LEA结构域，表明它可能是一种新型的LEA基因。CaMF5仅在第7和第8阶段的花蕾中和雄性可育系的开放花中表达，而在雄性不育系的任何受检器官中均未表达。此外，CaMF5在雄性可育系的花药中显示出最高的转录本丰度，而在其他任何检查过的器官（如萼片，花瓣，雌蕊，根，茎或叶）中均未检测到表达。总之，我们的研究结果表明，CaMF5是可能编码一种新型的LEA蛋白的相关花药和/或花粉发育花药特异性基因辣椒。