Abstract Background Oleaginous yeasts can be grown on different carbon sources, including lignocellulosic hydrolysate containing a mixture of glucose and xylose. However, not all yeast strains can utilize both the sugars for lipogenesis. Therefore, in this study, efforts were made to isolate dual sugar-utilizing oleaginous yeasts from different sources. Results A total of eleven isolates were obtained, which were screened for their ability to utilize various carbohydrates for lipogenesis. One promising yeast isolate Trichosporon mycotoxinivorans S2 was selected based on its capability to use a mixture of glucose and xylose and produce 44.86 ± 4.03% lipids, as well as its tolerance to fermentation inhibitors. In order to identify an inexpensive source of sugars, nondetoxified paddy straw hydrolysate (saccharified with cellulase), supplemented with 0.05% yeast extract, 0.18% peptone, and 0.04% MgSO4 was used for growth of the yeast, resulting in a yield of 5.17 g L−1 lipids with conversion productivity of 0.06 g L−1 h−1. Optimization of the levels of yeast extract, peptone, and MgSO4 for maximizing lipid production using Box–Behnken design led to an increase in lipid yield by 41.59%. FAME analysis of single cell oil revealed oleic acid (30.84%), palmitic acid (18.28%), and stearic acid (17.64%) as the major fatty acids. Conclusion The fatty acid profile illustrates the potential of T. mycotoxinivorans S2 to produce single cell oil as a feedstock for biodiesel. Therefore, the present study also indicated the potential of selected yeast to develop a zero-waste process for the complete valorization of paddy straw hydrolysate without detoxification. How to cite: Sagia S, Sharma A, Singh S, et al. Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw. Electronic Journal of Biotechnology 2020;44. https://doi.org/10.1016/j.ejbt.2020.01.009 .

中文翻译：

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一种新型酵母 Trichosporon mycotoxinivorans 的单细胞油生产用于稻草的完全和生态友好的增值

摘要背景含油酵母可以在不同的碳源上生长，包括含有葡萄糖和木糖混合物的木质纤维素水解物。然而，并非所有酵母菌株都可以利用这两种糖进行脂肪生成。因此，在本研究中，努力从不同来源分离双重利用糖的含油酵母。结果 共获得 11 个分离株，筛选它们利用各种碳水化合物进行脂肪生成的能力。选择了一种有前途的酵母分离株 Trichosporon mycotoxinivorans S2，因为它能够使用葡萄糖和木糖的混合物并产生 44.86 ± 4.03% 的脂质，以及对发酵抑制剂的耐受性。为了确定糖的廉价来源，未解毒的稻草水解物（用纤维素酶糖化），补充有 0.05% 酵母提取物、0.18% 蛋白胨和 0.04% MgSO4 用于酵母生长，产生 5.17 g L-1 脂质，转化率为 0.06 g L-1 h-1。使用 Box-Behnken 设计优化酵母提取物、蛋白胨和 MgSO4 的水平以最大化脂质产量，导致脂质产量增加 41.59%。单细胞油的 FAME 分析显示油酸 (30.84%)、棕榈酸 (18.28%) 和硬脂酸 (17.64%) 作为主要脂肪酸。结论 脂肪酸谱说明了 T. mycotoxinivorans S2 生产单细胞油作为生物柴油原料的潜力。因此，本研究还表明，选定的酵母有潜力开发一种零浪费工艺，在不解毒的情况下使稻草水解物完全增值。如何引用：Sagia S，夏尔马 A、辛格 S 等。一种新型酵母 Trichosporon mycotoxinivorans 的单细胞油生产，用于稻草的完全和生态友好的增值。生物技术电子杂志 2020；44。https://doi.org/10.1016/j.ejbt.2020.01.009。