Introduction

Since Populus has veritable value as timber, plywood, pulp, and paper, genomic research should create the sound basis for further breeding toward desirable wood quality attributes.

Materials and methods

In this study, we addressed the need for a research methodology that initially identifies and then characterize candidate genes encoding enzymes with wood property phenotypic traits, toward the aim of developing a genomics-based breeding technology.

Results

On 23 different poplar species/hybrid samples, we successfully amplified 55 primers designed on Populus trichocarpa L. Considering the number of polymorphic sites, out of 73,206 bp, 51 SNPs and 31 indel events were found. Non-synonymous single base mutations could be detected in number of 30, 21 out of 164 sequences were the number of minimum recombination events and 41 significant pairwise comparisons between loci could be detected.

Discussion and conclusion

Our results provide a roadmap for a future association genetic study between nucleotide diversity and precise evaluation of phenotype.

中文翻译：

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与栽培杨树（杨树）木材特性相关的候选基因的等位变异

介绍

由于杨树作为木材、胶合板、纸浆和纸张具有名副其实的价值，基因组研究应该为进一步培育理想的木材质量属性奠定良好的基础。

材料和方法

在这项研究中，我们解决了对研究方法的需求，该方法最初确定并表征编码具有木材特性表型特征的酶的候选基因，以开发基于基因组学的育种技术。

结果

在 23 个不同的杨树物种/杂种样品中，我们成功扩增了 55 个针对毛果杨设计的引物。考虑到多态位点的数量，在 73,206 bp 中，发现了 51 个 SNP 和 31 个插入缺失事件。在30个中可以检测到非同义单碱基突变，164个序列中的21个是最小重组事件的数量，并且可以检测到41个基因座之间的显着成对比较。

讨论与结论

我们的结果为未来核苷酸多样性与表型精确评估之间的关联遗传研究提供了路线图。