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Bioprinting and in vitro characterization of alginate dialdehyde–gelatin hydrogel bio-ink
Bio-Design and Manufacturing ( IF 7.9 ) Pub Date : 2020-01-23 , DOI: 10.1007/s42242-020-00058-8
Fu You , Xia Wu , Michael Kelly , Xiongbiao Chen

Cell-laden cardiac patches have recently been emerging to renew cellular sources for myocardial infarction (MI, commonly know as a heart attack) repair. However, the fabrication of cell-laden patches with porous structure remains challenging due to the limitations of currently available hydrogels and existing processing techniques. The present study utilized a bioprinting technique to fabricate hydrogel patches and characterize them in terms of printability, mechanical and biological properties. Cell-laden hydrogel (or bio-ink) was formulated from alginate dialdehyde (ADA) and gelatin (GEL) to improve the printability, degradability as well as bioactivity. Five groups of hydrogel compositions were designed to investigate the influence of the oxidation degree of ADA and hydrogels concentration on the properties of printed scaffolds. ADA–GEL hydrogels have generally shown favorable for living cells (EA.hy926 cells and hybrid human umbilical vein endothelial cell line). The hydrogel with an oxidation degree of 10% and a concentration ratio of 70/30 (or 10%ADA70–GEL30) demonstrated the best printability among the groups examined. Formulated hydrogels were also bioprinted with the living cells (EA.hy926), and the scaffolds printed were then subject to the cell culture for 7 days. Our results illustrate that the scaffolds bioprinted from 10%ADA70–GEL30 hydrogels had the best homogenous cell distribution and also the highest cell viability. Taken together, in the present study we synthesized a newly formulated bio-ink from ADA and GEL and for the fist time, used them to bioprint cardiac patches, which have the potential to be used in MI repair.

中文翻译:

藻酸盐二醛-明胶水凝胶生物油墨的生物印迹和体外表征

最近出现了充满细胞的心脏补丁,以更新用于心肌梗死(MI,通常称为心脏病发作)修复的细胞来源。然而,由于当前可用的水凝胶和现有加工技术的局限性,具有多孔结构的充满细胞的贴剂的制造仍然具有挑战性。本研究利用生物印刷技术来制造水凝胶贴剂,并在可印刷性,机械和生物学特性方面对其进行表征。由藻酸盐二醛(ADA)和明胶(GEL)配制载有细胞的水凝胶(或生物墨水),以改善可印刷性,降解性和生物活性。设计五组水凝胶组合物以研究ADA的氧化度和水凝胶浓度对印刷支架性能的影响。ADA-GEL水凝胶通常显示出对活细胞(EA.hy926细胞和人脐静脉内皮细胞混合细胞)有利。氧化度为10%,浓度比为70/30(或10%ADA70–GEL30)的水凝胶在所考察的组中显示出最佳的可印刷性。配制的水凝胶也用活细胞(EA.hy926)进行生物印刷,然后将印刷的支架进行细胞培养7天。我们的结果表明,从10%ADA70–GEL30水凝胶进行生物印染的支架具有最佳的均匀细胞分布和最高的细胞活力。综上所述,在本研究中,我们从ADA和GEL合成了一种新配制的生物墨水,并在第一时间将其用于生物打印心脏斑块,这些斑块具有用于MI修复的潜力。
更新日期:2020-01-23
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