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A de novo transcriptomic approach to study the influence of marine water depth in Macrocystis pyrifera alginate production
Aquatic Botany ( IF 1.8 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.aquabot.2020.103211
Sujay Paul , Erika Salavarría , Patricia Gil-Kodaka , Gretty K. Villena

Brown algae or brown seaweed is a commercial source of different polysaccharides/hydrocolloids including alginates and fucoidans. To increase the knowledge of transcript diversity and to understand the molecular pattern of alginate biosynthesis in brown seaweeds as influenced by tidal cycles, two RNA libraries were analyzed from Macrocystis pyrifera collected at the intertidal (0 m depth) and subtidal (10 m depth) zones. Yield and viscosity of sodium alginate were measured and scanning electron microscopy, as well as X-ray energy dispersion analysis of the sample materials were performed. Results revealed that frond samples from 10 m depth significantly produced more sodium alginate than that of 0 m depth. Corroborating the alginate yield data RNAseq analysis disclosed the overexpression of 8 alginate biosynthesis genes such as mannose-6-phosphate isomerase, phosphomannomutase, GDP-mannose 6-dehydrogenase, mannuronan C-5-epimerase, etc. at 10 m samples. Scanning electron microscopy study revealed the presence of alginate in the form of several trabecular structures in the meristematic cells of 10 m samples while the meristematic cells of 0 m samples were mostly found empty. To the best of our knowledge, this is the first transcriptome-based report of brown macroalga alginate production at different marine water depth.



中文翻译:

一个从头转录的方法来研究海水深度的影响Macrocystis pyrifera海藻酸钠生产

褐藻或褐藻是包括藻酸盐和岩藻依聚糖的不同多糖/水胶体的商业来源。为了增加转录本多样性的知识,并了解潮汐周期对褐藻中藻酸盐生物合成的分子模式的影响,分析了两个来自大果孢藻的RNA库收集在潮间带(0 m深度)和潮下带(10 m深度)区域。测量海藻酸钠的产率和粘度,并进行扫描电子显微镜以及样品材料的X射线能量色散分析。结果表明,深度为10 m的叶状样品产生的藻酸钠明显大于深度为0 m的叶藻酸钠。证实藻酸盐产量数据RNAseq分析揭示了8种藻酸盐生物合成基因的过表达,例如甘露糖6-磷酸异构酶,磷酸甘露糖异位酶,GDP-甘露糖6-脱氢酶,甘露糖醛酸C-5-表异构酶等。在10 m样本处 扫描电子显微镜研究发现,在10 m样品的分生细胞中,藻酸盐以几种小梁结构的形式存在,而0 m样品的分生细胞大多为空。据我们所知,这是第一份基于转录组的报告,显示了在不同海水深度下褐藻海藻酸盐的产量。

更新日期:2020-01-23
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