T cells provide critical immune surveillance to the central nervous system (CNS), and the cerebrospinal fluid (CSF) is thought to be a main route for their entry. Further characterization of the state of T cells in the CSF in healthy individuals is important for understanding how T cells provide protective immune surveillance without damaging the delicate environment of the CNS and providing tissue-specific context for understanding immune dysfunction in neuroinflammatory disease. Here, we have profiled T cells in the CSF of healthy human donors and have identified signatures related to cytotoxic capacity and tissue adaptation that are further exemplified in clonally expanded CSF T cells. By comparing profiles of clonally expanded T cells obtained from the CSF of patients with multiple sclerosis (MS) and healthy donors, we report that clonally expanded T cells from the CSF of patients with MS have heightened expression of genes related to T cell activation and cytotoxicity.

T 细胞为中枢神经系统 (CNS) 提供重要的免疫监视，而脑脊液 (CSF) 被认为是它们进入的主要途径。对健康个体脑脊液中 T 细胞状态的进一步表征对于了解 T 细胞如何提供保护性免疫监视而不破坏中枢神经系统的微妙环境以及为了解神经炎症性疾病中的免疫功能障碍提供组织特异性背景非常重要。在这里，我们对健康人类供体的 CSF 中的 T 细胞进行了分析，并确定了与细胞毒性能力和组织适应相关的特征，这些特征在克隆扩增的 CSF T 细胞中得到了进一步的例证。通过比较从多发性硬化症 (MS) 患者和健康供体的 CSF 中获得的克隆扩增 T 细胞的概况，