A 12-y-old spayed female Schipperke dog with a previous diagnosis of inflammatory bowel disease was presented with a 2-mo history of severe colitis. The patient’s condition progressed to hepatopathy, pneumonia, and dermatitis following management with prednisolone and dexamethasone sodium phosphate. Colonic biopsies identified severe necrosuppurative colitis with free and intracellular parasitic zoites. Postmortem examination confirmed extensive chronic-active ulcerative colitis, severe acute necrotizing hepatitis and splenitis, interstitial pneumonia, ulcerative dermatitis, myelitis (bone marrow), and mild meningoencephalitis with variable numbers of intracellular and extracellular protozoal zoites. PCR on samples of fresh colon was positive for Neospora caninum. Immunohistochemistry identified N. caninum tachyzoites in sections of colon, and a single tissue cyst in sections of brain. Administration of immunosuppressive drugs may have allowed systemic dissemination of Neospora from the intestinal tract.

一只 12 岁已绝育的雌性 Schipperke 犬先前诊断为炎症性肠病，有 2 个月的严重结肠炎病史。在使用泼尼松龙和地塞米松磷酸钠治疗后，患者的病情进展为肝病、肺炎和皮炎。结肠活检发现严重的坏死性化脓性结肠炎，伴有游离的和细胞内寄生的 zoites。尸检证实广泛的慢性活动性溃疡性结肠炎、严重的急性坏死性肝炎和脾炎、间质性肺炎、溃疡性皮炎、脊髓炎（骨髓）和轻度脑膜脑炎，具有不同数量的细胞内和细胞外原生动物zoites。新鲜结肠样本的 PCR 对犬新孢子虫呈阳性反应。免疫组化鉴定N. caninum tachyzoites 在结肠切片中，在大脑切片中为单个组织囊肿。免疫抑制药物的施用可能允许新孢子虫从肠道系统性传播。