2-Aminobenzoic acid and heterocyclic amino acids are aromatic homologues of β-amino acids, but their chemical properties are quite distinct. Because of the poor nucleophilicity of the amino group conjugated with the aromatic ring, no literature reports of their successful ribosomal elongation in nascent peptide chains have appeared to date. Here we report for the first time their incorporation in nascent peptide chains by means of a reconstituted translation system in which a designer tRNAPro1E2 capable of recruiting the elongation factors EF-Tu and EF-P was charged with their derivatives and the corresponding peptides were successfully expressed. We also demonstrate the expression of macrocyclic peptides containing exotic amino acids, including aminobenzoic acid derivatives.

中文翻译：

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氨基苯甲酸衍生物的核糖体延伸

2-氨基苯甲酸和杂环氨基酸是β-氨基酸的芳香族同系物，但它们的化学性质截然不同。由于与芳环共轭的氨基的亲核性较差，迄今为止还没有文献报道它们在新生肽链中成功的核糖体延伸。在这里，我们首次报告了它们通过重建的翻译系统掺入新生肽链中，其中能够招募延伸因子 EF-Tu 和 EF-P 的设计者 tRNAPro1E2 被加载到它们的衍生物中，并成功表达了相应的肽. 我们还展示了含有外来氨基酸（包括氨基苯甲酸衍生物）的大环肽的表达。