Anoplophora glabripennis (Motschulsky, 1853), native to eastern Asia, is a destructive woodborer of many ornamental species, leading to the decline and the death of the attacked trees. In outbreak areas as Europe or North America, this pest is usually identified using morphological or molecular analyses of adult or larval specimens. However, the procedures for collecting A. glabripennis specimens from infested plants are too expensive and time consuming for routine screening. A noninvasive diagnostic tool based on frass discrimination is therefore crucial for the rapid identification of A. glabripennis at different development stages in the host. This article describes a rapid diagnostic protocol based on loop-mediated isothermal amplification (LAMP). DNA extracted from A. glabripennis frass was amplified with both visual and real-time LAMP and compared with those of nontarget species. The results show that the method is reliable and accurate and therefore could be a promising diagnostic tool in phytosanitary surveys.

中文翻译：

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环介导等温扩增法从 Frass 中鉴定 Anoplophora glabripennis（鞘翅目：天牛科）

Anoplophora glabripennis (Motschulsky, 1853) 原产于东亚，是许多观赏物种的破坏性蛀木虫，导致受攻击树木的衰退和死亡。在欧洲或北美等暴发地区，通常通过对成虫或幼虫标本进行形态学或分子分析来识别这种有害生物。然而，从受侵染的植物中收集光肩草标本的程序对于常规筛选来说过于昂贵和耗时。因此，基于 fras 鉴别的非侵入性诊断工具对于快速识别宿主不同发育阶段的光秃菌至关重要。本文介绍了一种基于环介导等温扩增 (LAMP) 的快速诊断协议。从 A 中提取的 DNA。glabripennis frass 用视觉和实时 LAMP 放大，并与非目标物种进行比较。结果表明，该方法可靠且准确，因此可能成为植物检疫调查中很有前景的诊断工具。