Busseola fusca (Fuller) (Lepidoptera: Noctuidae), the maize stalk borer, is a widespread crop pest in sub-Saharan Africa that has been the focus of biological research and intensive management strategies. Here, we present a comprehensive annotated transcriptome of B. fusca (originally collected in the Western Province of Kenya) based on ten pooled libraries including a wide array of developmental stages, tissue types, and exposures to parasitoid wasps. Parasitoid wasps have been used as a form of biocontrol to try and reduce crop losses with variable success, in part due to differential infectivities and immune responses among wasps and hosts. We identified a number of loci of interest for pest management, including genes potentially involved in chemoreception, immunity, and response to insecticides. The comprehensive sampling design used expands our current understanding of the transcriptome of this species and deepens the list of potential target genes for future crop loss mitigation, in addition to highlighting candidate loci for differential expression and functional genetic analyses in this important pest species.

中文翻译：

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来自多种组织类型、发育阶段和寄生蜂暴露的玉米茎螟的综合转录组，Busseola fusca。

Busseola fusca (Fuller)（鳞翅目：夜蛾科）是一种玉米螟虫，是撒哈拉以南非洲广泛存在的作物害虫，一直是生物研究和集约化管理战略的重点。在这里，我们展示了B. fusca的综合注释转录组（最初收集于肯尼亚西部省）基于十个汇集库，包括各种发育阶段、组织类型和寄生蜂的暴露情况。寄生蜂已被用作一种生物防治形式，以尝试减少作物损失并取得不同程度的成功，部分原因是黄蜂和宿主之间的感染性和免疫反应不同。我们确定了许多害虫管理感兴趣的基因座，包括可能涉及化学感受、免疫和对杀虫剂的反应的基因。所使用的综合抽样设计扩展了我们目前对该物种转录组的理解，并加深了未来减轻作物损失的潜在目标基因列表，