Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together by an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms underlying the stability of microtubule triplets, as well as centriole architectural integrity remain poorly understood. Here, using ultrastructure expansion microscopy for nanoscale protein mapping, we reveal that POC16 and its human homolog WDR90 are components of the microtubule wall along the central core region of the centriole. We further found that WDR90 is an evolutionary microtubule associated protein. Finally, we demonstrate that WDR90 depletion impairs the localization of inner scaffold components, leading to centriole structural abnormalities in human cells. Altogether, this work highlights that WDR90 is an evolutionary conserved molecular player participating in centriole architecture integrity.

中文翻译：

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WDR90 是一种对中心粒结构完整性很重要的中心微管壁蛋白

中心粒的特征是由内部蛋白质支架固定在一起的微管三联体的九重排列。这些结构坚固的细胞器在执行其功能时会经历剧烈的细胞过程，例如细胞分裂或纤毛跳动。然而，微管三联体稳定性以及中心粒结构完整性的分子机制仍然知之甚少。在这里，使用超微结构扩展显微镜进行纳米级蛋白质作图，我们发现 POC16 及其人类同源物 WDR90 是沿中心粒中央核心区域的微管壁的组成部分。我们进一步发现 WDR90 是一种进化微管相关蛋白。最后，我们证明 WDR90 耗尽会损害内部支架组件的定位，导致人体细胞中心粒结构异常。总之，这项工作强调 WDR90 是参与中心粒结构完整性的进化保守分子参与者。