Salinity stress is an important plant growth limiting factor influencing crop productivity negatively. Microbial interventions for salinity stress mitigation have invited significant attention due to the promising impacts of interactive associations on the intrinsic mechanisms of plants. We report the impact of microbial inoculation of a halotolerant methylotrophic actinobacterium (Nocardioides sp. NIMMe6; LC140963) and seed coating of its phytohormone-rich bacterial culture filtrate extract (BCFE) on wheat seedlings grown under saline conditions. Different plant-growth-promoting (PGP) attributes of the bacterium in terms of its growth in N-limiting media and siderophore and phytohormone [indole-3-acetic acid (IAA) and salicylic acid] production influenced plant growth positively. Microbial inoculation and priming with BCFE resulted in improved germination (92% in primed seeds at 10 dS m^–1), growth, and biochemical accumulation (total protein 42.01 and 28.75 mg g^–1 in shoot and root tissues at 10 dS m^–1 in BCFE-primed seeds) and enhanced the activity level of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase) to confer stress mitigation. Biopriming with BCFE proved impactful. The BCFE application has further influenced the overexpression of defense-related genes in the seedlings grown under salinity stress condition. Liquid chromatography–mass spectrometry-based characterization of the biomolecules in the BCFE revealed quantification of salicylate and indole-3-acetate (Rt 4.978 min, m/z 138.1 and 6.177 min, 129.1), respectively. The high tolerance limit of the bacterium to 10% NaCl in the culture media suggested its possible survival and growth under high soil salinity condition as microbial inoculant. The production of a high quantity of IAA (45.6 μg ml^–1 of culture filtrate) by the bacterium reflected its capability to not only support plant growth under salinity condition but also mitigate

stress due to the impact of phytohormone as defense mitigators. The study suggested that although microbial inoculation offers stress mitigation in plants, the phytohormone-rich BCFE from Nocardioides sp. NIMMe6 has potential implications for defense against salinity stress in wheat.

盐分胁迫是重要的植物生长限制因素，对作物生产力产生负面影响。由于互动协会对植物内在机制的潜在影响，用于减轻盐分胁迫的微生物干预引起了极大的关注。我们报告了微生物接种的耐盐甲基营养型放线放线菌（诺卡氏菌sp。NIMMe6; （LC140963）并将其富含植物激素的细菌培养滤液提取物（BCFE）的种子包被在盐渍条件下生长的小麦幼苗上。就其在氮限制培养基中的生长以及铁载体和植物激素[吲哚-3-乙酸（IAA）和水杨酸]的生长而言，该细菌具有不同的植物生长促进（PGP）属性，对植物生长产生了积极影响。微生物接种，并用吸BCFE导致改善的萌发（在10个德尚在浸过水的种子92％M ^-1），生长，和生化积累（总蛋白42.01和28.75毫克克^-1枝条和根组织在10个德尚中号^-1并增强了抗氧化酶（超氧化物歧化酶，过氧化氢酶，过氧化物酶和抗坏血酸过氧化物酶）的活性水平，从而减轻了胁迫。BCFE的生物引发被证明是有效的。BCFE的应用进一步影响了盐分胁迫条件下幼苗中防御相关基因的过表达。液相色谱-质谱法对BCFE中的生物分子进行了表征，结果表明了水杨酸酯和吲哚-3-乙酸酯的定量（Rt 4.978分钟，m / z138.1和6.177分钟，129.1）。该细菌对培养基中10％NaCl的高耐受极限表明，在高土壤盐分条件下，该细菌可能作为微生物接种剂存活和生长。细菌产生大量的IAA（45.6μgml ^–1培养滤液）反映了其不仅支持盐度条件下植物生长的能力，而且还减轻了

由于植物激素作为防御缓解剂的影响而产生的压力。研究表明，尽管接种微生物可以缓解植物的压力，但富含植物激素的BCFE来自诺卡氏菌sp。NIMMe6对于防御小麦盐分胁迫具有潜在的意义。