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Correction: The RNA-binding protein HuD regulates autophagosome formation in pancreatic β cells by promoting autophagy-related gene 5 expression.
Journal of Biological Chemistry ( IF 5.5 ) Pub Date : 2020-09-18 , DOI: 10.1074/jbc.aac120.015676
Chongtae Kim , Wook Kim , Heejin Lee , Eunbyul Ji , Yun-Jeong Choe , Jennifer L. Martindale , Wado Akamatsu , Hideyuki Okano , Ho-Shik Kim , Suk Woo Nam , Myriam Gorospe , Eun Kyung Lee

VOLUME 289 (2013) PAGES 112–121The autophagy flux experiments shown in Figs. 2D and 4A were performed similarly, either by serial transfection of pcDNA or pHuD followed by transfection with the GFP reporter construct or by single transfection of pcDNA or pHuD. No difference was observed with either method. As no difference was observed, the HuD and actin immunoblots from Fig. 2D were mistakenly used in Fig. 4A. The corrected Fig. 4A now shows results from a single transfection rather than a serial transfection. The quantification has also been corrected. This correction does not affect the results or conclusions of this work.jbc;295/38/13408/F4F1F4Figure 4A

中文翻译:

校正:RNA结合蛋白HuD通过促进自噬相关基因5的表达来调节胰腺β细胞中自噬体的形成。

卷289(2013)第112–121页自噬通量实验如图1和图2所示。通过连续转染pcDNA或pHuD,然后用GFP报告基因构建体转染,或通过pcDNA或pHuD的单次转染,类似地进行2D和4A。两种方法均未观察到差异。由于未观察到差异,因此图4D中错误地使用了图2D的HuD和肌动蛋白免疫印迹。校正后的图4A现在显示了单次转染而不是连续转染的结果。量化也已更正。该更正不影响这项工作的结果或结论。jbc; 295/38/13408 / F4F1F4图4A
更新日期:2020-09-20
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