Protein detection technologies are important for early disease diagnosis because of their advantages over biopsy testing. Considering the diagnostic potential of prostate‐specific antigen (PSA) in prostate cancer, in this study, we present a lateral flow immunoassay (LFIA) for successful detection of PSA by using silica‐coated CdSe@ZnS quantum dots (QD@SiO₂). The QD@SiO₂ were synthesized using a reverse microemulsion method. The average size of the synthesized nanoparticles (NPs) was 51.76 nm, and their quantum yield (QY) was relatively high (36.5%). PSA detection was performed using LFIA on a strip. The QD@SiO₂ surface was modified by conjugating the anti‐PSA antibody to detect the PSA. At concentrations above 1.0754 ng/mL, PSA was detected using the designed LFIA system without any nonspecific binding. The proposed QD@SiO₂ has other bioapplications as well.

中文翻译：

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硅胶包裹的CdSe @ ZnS量子点的前列腺特异性抗原的横向流动免疫测定

蛋白质检测技术相对于活检测试具有优势，因此对于早期疾病诊断非常重要。考虑到前列腺特异性抗原（PSA）在前列腺癌中的诊断潜力，在本研究中，我们提出了一种侧向流免疫测定（LFIA），可通过使用二氧化硅涂层的CdSe @ ZnS量子点（QD @ SiO ₂）成功检测PSA。。使用反相微乳液法合成了QD @ SiO ₂。合成的纳米粒子（NPs）的平均大小为51.76 nm，其量子产率（QY）相对较高（36.5％）。使用试纸上的LFIA进行PSA检测。QD @ SiO ₂通过缀合抗PSA抗体检测PSA，对表面进行修饰。在高于1.0754 ng / mL的浓度下，使用设计的LFIA系统检测到PSA，没有任何非特异性结合。拟议的QD @ SiO ₂也具有其他生物应用。