Histopathologic surgical margin assessment in veterinary patients is an imprecise science with assessment limited to a small proportion of the surgical margin due to time and finances. Incomplete excision of canine mast cell tumours (MCTs) alters treatment recommendations and prognosis. Optical coherence tomography (OCT) is a novel imaging modality that has been reported in a single veterinary study for surgical margin assessment. Twenty-five dogs with 34 MCTs were enrolled in a prospective pilot-study to assess the imaging characteristics of canine MCTs with OCT and to evaluate the feasibility and utility of OCT-guided histopathology. All dogs underwent routine surgical excision of MCTs. OCT imaging was used to assess the entire surgical margin prior to placement in formalin. Either normal areas or areas suspected of incomplete MCT excision were inked. Standard histopathologic sectioning and tangential sectioning of inked areas were performed and compared to OCT results. OCT identified MCT near the surgical margin in 10 of 26 specimens (38.4%). Four specimens suspicious for incomplete margins on OCT had incomplete MCT excision that was missed on standard histopathologic sectioning. Six specimens had OCT-guided sections taken as suspicious, which did not show MCT on histopathology. OCT-guided pathology sections were able to detect incompletely excised MCT near the surgical margin with a sensitivity of 90% and specificity of 56.2% in this preliminary study. OCT imaging shows promise for guiding pathologists to areas of interest to improve the diagnostic accuracy of surgical margin assessment in excised canine MCTs.

中文翻译：

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光学相干断层扫描定向组织病理学评估犬肥大细胞瘤手术切缘的可行性和实用性

兽医患者的组织病理学手术切缘评估是一门不精确的科学，由于时间和经济原因，评估仅限于手术切缘的一小部分。犬肥大细胞瘤 (MCT) 的不完全切除会改变治疗建议和预后。光学相干断层扫描 (OCT) 是一种新颖的成像方式，已在一项用于手术切缘评估的兽医研究中得到报道。25 只拥有 34 个 MCT 的狗被纳入一项前瞻性试点研究，以评估犬 MCT 与 OCT 的成像特征，并评估 OCT 引导的组织病理学的可行性和实用性。所有狗都接受了 MCT 的常规手术切除。OCT 成像用于在放置福尔马林之前评估整个手术切缘。正常区域或怀疑 MCT 切除不完全的区域都被涂上了墨水。进行了墨迹区域的标准组织病理学切片和切向切片，并与 OCT 结果进行了比较。OCT 在 26 个标本中的 10 个 (38.4%) 中识别出手术切缘附近的 MCT。四个怀疑 OCT 边缘不完整的标本具有不完整的 MCT 切除，标准组织病理学切片遗漏了这些样本。六个标本的 OCT 引导切片被视为可疑，在组织病理学上未显示 MCT。在这项初步研究中，OCT 引导的病理切片能够检测到手术切缘附近未完全切除的 MCT，灵敏度为 90%，特异性为 56.2%。OCT 成像有望指导病理学家关注感兴趣的区域，以提高切除的犬 MCT 手术切缘评估的诊断准确性。进行了墨迹区域的标准组织病理学切片和切向切片，并与 OCT 结果进行了比较。OCT 在 26 个标本中的 10 个 (38.4%) 中识别出手术切缘附近的 MCT。四个怀疑 OCT 边缘不完整的标本具有不完整的 MCT 切除，标准组织病理学切片遗漏了这些样本。六个标本的 OCT 引导切片被视为可疑，在组织病理学上未显示 MCT。在这项初步研究中，OCT 引导的病理切片能够检测到手术切缘附近未完全切除的 MCT，灵敏度为 90%，特异性为 56.2%。OCT 成像有望指导病理学家关注感兴趣的区域，以提高切除的犬 MCT 手术切缘评估的诊断准确性。进行了墨迹区域的标准组织病理学切片和切向切片，并与 OCT 结果进行了比较。OCT 在 26 个标本中的 10 个 (38.4%) 中识别出手术切缘附近的 MCT。四个怀疑 OCT 边缘不完整的标本具有不完整的 MCT 切除，标准组织病理学切片遗漏了这些样本。六个标本的 OCT 引导切片被视为可疑，在组织病理学上未显示 MCT。在这项初步研究中，OCT 引导的病理切片能够检测到手术切缘附近未完全切除的 MCT，灵敏度为 90%，特异性为 56.2%。OCT 成像有望指导病理学家关注感兴趣的区域，以提高切除的犬 MCT 手术切缘评估的诊断准确性。