The symbiotic association between plant roots and arbuscular mycorrhizal fungi (AMF) promotes plant growth, and reduces toxic metals accumulation in plants. In this study, the effect of inoculation with indigenous AMF (Glomus Spp.) and co-application of AMF and nano-TiO₂ on Cd accumulation on activities of stress enzymes in cowpea at pre-flowering stage was examined. Soil substrates were spiked with either Cd (10 mg/kg) and inoculated with either AMF suspension (7 ml) or AMF+nTiO₂ (200 mg/kg). The results showed that inoculation with AMF and AMF+nTiO₂ promoted germination and chlorophyll contents by at least 12% and 45%, respectively. As envisaged, AMF+nTiO₂ reduced uptake and decreased translocation of Cd to above-ground biomass better than inoculation solely with AMF. In the aspect of Cd toxicity assessed by enzymes activity, sole AMF inoculation and AMF+nTiO₂ had varied effects on the stress enzymes activity. However, nTiO₂ interacted synergistically with AMF to improve stress tolerance by reducing the lipid peroxidation in plants (in term of the amount of malondialdehyde) more than sole inoculation with AMF. In conclusion, the present study demonstrated that there is stimulatory effect of nano-TiO₂ on the activity of AMF in alleviating Cd stress in pre-flowering cowpea.

植物根与丛枝菌根真菌（AMF）之间的共生关系促进植物生长，并减少植物中有毒金属的积累。在这项研究中，研究了在开花前，用原生AMF（Glomus Spp。）接种以及AMF和纳米TiO ₂共同施用对镉积累对cow豆胁迫酶活性的影响。用Cd（10 mg / kg）掺加土壤基质，并接种AMF悬浮液（7 ml）或AMF + n TiO ₂（200 mg / kg）。结果表明，接种AMF和AMF + n TiO _2分别使发芽和叶绿素含量分别提高至少12％和45％。按照设想，AMF + n TiO₂比单独接种AMF更好地减少了Cd的吸收并减少了Cd向地上生物量的转移。在通过酶活性评估镉的毒性方面，单独的AMF接种和AMF + n TiO ₂对胁迫酶活性的影响各不相同。但是，n TiO ₂与AMF协同相互作用，通过减少植物中脂质的过氧化作用（以丙二醛的量计），比单独接种AMF更好，从而提高了耐逆性。综上所述，本研究表明纳米TiO ₂对AMF活性具有缓解effect花豆pea镉胁迫的活性。