Production of algae biomass from wastewaters have been considered as an economical strategy for feasible commercialization of microalgae industry. Technically, large scale cultivation of microalgae in wastewaters should be conducted in semi-continuous and/or continuous modes, in which stable health of algal cultures of previous generations for the use of later generations needs to be ensured. In this regards, this study has investigated stability of growth and pollutant removal capability of Chlorella variabilis TH03-bacteria consortia using domestic wastewater as the sole of nutrient source in raceway ponds. Experiments were performed in semi-continuous mode with the aim of recovering valuable nutrients including carbon, nitrogen and phosphorous from the waste stream to algal biomass. Data revealed that prior domestication of C. variabilis TH03 in unsterilized domestic wastewaters established seed cultures of C. variabilis TH03-bacteria consortia, which enabled to grow effectively in new wastewaters under highly variable conditions. Moreover, once nutrient levels dropped below those required by the national technical regulation on domestic wastewater QCVN 14 : 2008/BTNMT, the replacement of 80% volume of the algal-bacteria culture with new wastewater media was recommended to ensure both culture stability as well as high pollutant treatment capacity of the raceway systems. Under the optimal replacement, biomass concentration and areal biomass productivity of 1.67–1.85 g/L and 11.1–15.3 g/m² day, respectively, were steadily achieved over 148 days of the outdoor cultivations. Removal efficiencies of nutrients in the form of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorous (TP) removed by the C. variabilis TH03-bacteria consortia in the reactor systems were determined as 64.7–90.7, 85.1–96.8 and 99.7%–100%, respectively. The obtained results are useful for further optimization including design and manage C. variabilis TH03-bacteria consortia based continuous processes in larger scales for the production of high volume and low price biomass, helping to obtain returns from treatment of the waste stream, which is currently considered as a major source of water pollution in Vietnam.

从废水中生产藻类生物质已被认为是微藻类工业可行商业化的经济策略。从技术上讲，废水中微藻的大规模培养应以半连续和/或连续方式进行，其中需要确保前代藻类培养物的稳定健康，以供后代使用。因此，本研究调查了变异小球藻的生长稳定性和污染物去除能力。TH03-细菌联合体，使用生活污水作为跑道池中唯一的营养来源。以半连续模式进行实验，目的是从废物流中回收有价值的养分，包括碳，氮和磷，以至藻类生物质。数据表明，现有的驯化C.变异在建立种子培养的未经消毒的生活污水TH03 C.变异TH03-细菌联合体，能够在高度可变的条件下在新废水中有效生长。此外，一旦营养水平降至国家生活用水QCVN 14：2008 / BTNMT的国家技术法规要求的水平以下，则建议用新的废水培养基替代80％体积的藻类细菌培养物，以确保培养物的稳定性和稳定性。滚道系统的高污染物处理能力。在最佳替代条件下， 在148天的室外栽培中，生物量浓度和面积生物量分别达到1.67-1.85 g / L和11.1-15.3 g / m ²天。营养物质以化学需氧量（COD），总氮（TN）和总磷（TP）的形式去除营养的效率C.栎在反应器系统TH03-细菌聚生体确定为分别64.7-90.7，85.1-96.8和99.7％-100％。获得的结果可用于进一步优化，包括设计和管理基于变异梭状芽胞杆菌TH03-细菌的大规模生产大批量和廉价生物质的连续流程，有助于从废物流的处理中获得回报。被认为是越南水污染的主要来源。