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Evolution of antigenic and genetic characteristics of foot-and-mouth disease virus serotype A circulating in Thailand, 2007-2019.
Virus Research ( IF 5 ) Pub Date : 2020-09-19 , DOI: 10.1016/j.virusres.2020.198166
Kingkarn Boonsuya Seeyo 1 , Tatsuya Nishi 2 , Rie Kawaguchi 2 , Sahawatchara Ungvanijban 1 , Romphruke Udon 1 , Katsuhiko Fukai 2 , Makoto Yamakawa 2 , Theera Rukkwamsuk 3
Affiliation  

Foot-and-mouth disease (FMD) is a persistent, major economic concern for livestock productivity, which is highly exacerbated by outbreaks in Thailand. FMD virus (FMDV) serotype A is more highly antigenic and genetically diverse than other serotypes, which has important implications for vaccine development as well as selection. Therefore, it is essential to continuously monitor antigenic and genetic changes of field isolates of FMDV serotype A. Here we used antisera against three vaccine strains (A/118/87, A/Sakolnakorn/97, and A/Lopburi/2012) to analyze the antigenicity of 133 field isolates of FMDV serotypes A in Thailand from 2007 to 2019. The majority of the isolates from 2007 to 2008 reacted only with the antiserum against strain A/118/87. In contrast, antigenic analysis revealed broad cross-reactivity and antigenic variations of the isolates from 2009 through 2019 against strains A/Sakolnakorn/97 and A/Lopburi/2012. These results indicate periodic changes in the antigenicity of field isolates of FMDV serotype A. Phylogenetic analysis of the VP1 region revealed that all isolates were of the Sea-97 lineage within the ASIA topotype. Analysis of the L-fragment genome sequences of 30 FMDV isolates collected throughout Thailand revealed highly variable amino acid sequences of VP1 and 3A, with the lowest average identity (94.56 %) and invariant (78.43 %) rates, respectively. The present findings indicate the importance of an active routine surveillance system incorporating antigenic and genetic analysis designated to continually update information about field isolates of FMDV serotype A. Such a system is essential for establishing and improving measures to control FMDV infections in Thailand and in neighboring Asian countries.



中文翻译:

2007-2019年泰国流行的口蹄疫病毒A血清型抗原和遗传特征的演变。

口蹄疫 (FMD) 是牲畜生产力持续存在的主要经济问题,泰国的暴发加剧了这种情况。口蹄疫病毒 (FMDV) 血清型 A 比其他血清型具有更高的抗原性和遗传多样性,这对疫苗开发和选择具有重要意义。因此,有必要持续监测 FMDV 血清型 A 型野外分离株的抗原和遗传变化。 这里我们使用针对三种疫苗株(A/118/87、A/Sakolnakorn/97 和 A/Lopburi/2012)的抗血清进行分析2007 年至 2019 年泰国 133 株 FMDV 血清型 A 的野外分离株的抗原性。2007 至 2008 年的大多数分离株仅与抗 A/118/87 株的抗血清反应。相比之下,抗原分析显示,从 2009 年到 2019 年,分离株对 A/Sakolnakorn/97 和 A/Lopburi/2012 菌株具有广泛的交叉反应性和抗原变异。这些结果表明 FMDV 血清型 A 的野外分离物抗原性的周期性变化。VP1 区域的系统发育分析表明,所有分离物都属于 ASIA 拓扑型内的 Sea-97 谱系。对整个泰国收集的 30 个 FMDV 分离株的 L 片段基因组序列的分析揭示了 VP1 和 3A 的高度可变氨基酸序列,分别具有最低的平均同一性 (94.56%) 和不变率 (78.43%)。目前的研究结果表明,一个积极的常规监测系统的重要性,该系统结合了抗原和遗传分析,旨在不断更新有关 A 型口蹄疫病毒现场分离株的信息。

更新日期:2020-10-11
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