Chrysin (5,7-dihydroxyflavone), a nutraceutical flavonoid present in diverse plants, has a backbone structure shared with the flavone backbone, with additional hydroxyl groups that confers its antioxidant properties and effects at the GABA_A receptor complex. However, whether these effects are due to the hydroxyl groups is unknown. Here we report the effects of chrysin or the flavone backbone (1 mg/kg) in rats subjected to the elevated plus-maze and the locomotor activity test, as well as in the zebrafish evaluated in light/dark model. Chrysin, but not flavone, increased entries and time in the open arms of the elevated plus-maze, as well as time on white compartment of the light/dark model in zebrafish. These effects were comparable to diazepam, and were devoid of motor effects in both tests, as well as in the locomotor activity test. On the other hand, flavone decreased risk assessment in the light/dark test but increased rearing in the locomotor activity test in rats, suggesting effects threat information gathering; important species differences suggest new avenues of research. It is suggested that the specific effects of chrysin in relation to flavone include more of a mechanism of action in which in addition to its action at the GABA_A/benzodiazepine receptor complex also could be involved its free radical scavenging abilities, which require specific research. Preprint: https://doi.org/10.1101/575514; Data and scripts:https://github.com/lanec-unifesspa/chrysin.

白杨素（5,7-二羟基黄酮）是一种存在于多种植物中的营养类黄酮，具有与黄酮骨架共享的骨架结构，并带有额外的羟基，赋予其抗氧化特性和对 GABA _{A 的作用}受体复合物。然而，这些影响是否由羟基引起尚不清楚。在这里，我们报告了白杨素或黄酮骨架 (1 mg/kg) 对经受高架十字迷宫和运动活动测试的大鼠以及在光/暗模型中评估的斑马鱼的影响。白杨素，但不是黄酮，增加了高架十字迷宫张开臂的条目和时间，以及斑马鱼亮/暗模型的白色隔间的时间。这些效果与地西泮相当，并且在两个测试和运动活动测试中都没有运动效果。另一方面，黄酮在光/暗测试中降低了风险评估，但在大鼠的运动活动测试中增加了饲养，表明影响威胁信息收集；重要的物种差异表明了新的研究途径。_一/苯二氮卓受体复合物还可以参与其清除自由基的能力，这需要具体研究。预印本：https://doi.org/10.1101/575514；数据和脚本： https : //github.com/lanec-unifesspa/chrysin。