The bacterium Xanthomonas oryzae pv. Oryzae (Xoo) causes blight in rice worldwide, resulting in significant crop loss. However, no gene underlying a quantitative trait locus (QTL) for resistance against Xoo has been cloned yet. Here, we report the map-based cloning of a QTL, in which the NBS8R gene confers quantitative resistance to Xoo. NBS8R encodes an NB-ARC protein, which is involved in pathogen/microbe-associated molecular pattern-triggered immunity and whose expression is regulated by non-TAL effector XopQ-inducible Osa-miR1876 through DNA methylation. Sequence analysis of NBS8R in wild rice species and rice cultivars suggests that the Osa-miR1876 binding sites in the 5′ UTR of NBS8R are inserted by chance and have undergone variations with Osa-miR1876 throughout evolution. The interaction between NBS8R and XopQ-inducible Osa-miR1876 is partially in keeping with the zigzag model, revealing that quantitative genes may also follow this model to control the innate immune response or basal disease resistance, and may prove valuable in utilizing the existing landraces that harbor the NBS8R gene but with no Osa-miR1876 binding site in rice breeding for bacterial blight resistance.

细菌Xanthomonas oryzae pv 。Oryzae（Xoo）在全世界的稻米中造成疫病，导致严重的农作物损失。但是，尚未克隆出对Xoo抗性的定量性状基因座（QTL）的基础基因。在这里，我们报告了基于QTL的基于图的克隆，其中NBS8R基因赋予Xoo定量抗性。NBS8R编码一种NB-ARC蛋白，它参与病原体/微生物相关的分子模式触发的免疫，其表达受非TAL效应子XopQ诱导的Osa-miR1876通过DNA甲基化的调控。NBS8R的序列分析在野生稻和栽培稻表明OSA-miR1876结合在5'UTR网站NBS8R偶然被插入并在整个进化与OSA-miR1876经历的变化。NBS8R 和XopQ诱导型Osa-miR1876之间的相互作用部分与之字形模型保持一致，这表明定量基因也可以遵循该模型来控制先天性免疫应答或基础疾病抗性，并且对于利用现有的地方品种可能证明是有价值的。心怀NBS8R基因但育种抗白叶枯病水稻无OSA-miR1876结合位点。