Decellularized extracellular matrix is one of the most promising biological scaffold supporting in vitro tissue growth and in vivo tissue regeneration in both preclinical research and clinical practice. In case of thick tissues or even organs, conventional static decellularization methods based on chemical or enzymatic treatments are not effective in removing the native cellular material without affecting the extracellular matrix. To overcome this limitation, dynamic decellularization methods, mostly based on perfusion and agitation, have been proposed. In this study, we developed a low-cost scalable 3D-printed sample-holder for agitation-based decellularization purposes, designed for treating multiple specimens simultaneously and for improving efficiency, homogeneity and reproducibility of the decellularization treatment with respect to conventional agitation-based approaches. In detail, the proposed sample-holder is able to house up to four specimens and, immersed in the decellularizing solution within a beaker placed on a magnetic stirrer, to expose them to convective flow, enhancing the solution transport through the specimens while protecting them. Computational fluid dynamics analyses were performed to investigate the fluid phenomena establishing within the beaker and to support the sample-holder design. Exploratory biological tests performed on human skin specimens demonstrated that the sample-holder reduces process duration and increases treatment homogeneity and reproducibility.

脱细胞细胞外基质是最有前途的支持体外组织生长和体内组织的生物支架之一临床前研究和临床实践中的组织再生。在厚组织甚至器官的情况下，基于化学或酶处理的传统静态脱细胞方法不能有效去除天然细胞材料而不影响细胞外基质。为了克服这一限制，已经提出了主要基于灌注和搅拌的动态脱细胞方法。在这项研究中，我们开发了一种低成本、可扩展的 3D 打印样品架，用于基于搅拌的脱细胞目的，旨在同时处理多个样本，并提高脱细胞处理的效率、均质性和可重复性相对于传统的基于搅拌的方法. 详细地说，建议的样品架最多可容纳四个样品，并且，浸入放置在磁力搅拌器上的烧杯内的脱细胞溶液中，使它们暴露于对流中，在保护样品的同时增强溶液通过样品的传输。进行计算流体动力学分析以研究在烧杯内建立的流体现象并支持样品架设计。对人体皮肤标本进行的探索性生物学测试表明，样品架缩短了过程持续时间并提高了治疗的均匀性和可重复性。进行计算流体动力学分析以研究在烧杯内建立的流体现象并支持样品架设计。对人体皮肤标本进行的探索性生物学测试表明，样品架缩短了过程持续时间并提高了治疗的均匀性和可重复性。进行计算流体动力学分析以研究在烧杯内建立的流体现象并支持样品架设计。对人体皮肤标本进行的探索性生物学测试表明，样品架缩短了过程持续时间并提高了治疗的均匀性和可重复性。