Antibacterial resistance has become a global threat, and there is a pressing need for new antibacterial agents to treat bacterial infection. Silver nanoparticles (AgNPs) covered with cationic carbosilane (CBS) dendrons are promising nanomaterials thanks to their antibacterial activity and their ability to deliver antibacterial agents like antimicrobial proteins (AMP); lysozyme and bacteriophage endolysin. However, without an understanding and control of the nature of interactions between endolysin and dendronized AgNPs and affinity of serum proteins to NPs surface, the application of this multifunctional system in medicine and biology is limited. Herein, we investigate the interactions of PEGylated and non-PEGylated CBS dendronized AgNPs (PEG-Dend-AgNPs and Dend-AgNPs, respectively) with KP27 endolysin, lysozyme and human serum albumin (HSA) to check the binding mechanism and affinity between the NPs and proteins and the role of the PEG chain on the surface of dendritic silver nanoparticles. Analysis of binding mechanism with thermophoresis, fluorescence, thermodynamics, SPR and adsorption shows that endolysin and lysozyme have a higher affinity for PEG-Dend-AgNPs, whereas HSA has higher affinity to Dend-AgNPs. Lysozyme and endolysin create a monolayer “protein-PEG-Dend-AgNPs” corona and HSA creates a multilayer “protein-Dend-AgNPs”. These phenomena help to us to understand how surface modification is involved in the mechanisms of interaction of dendronized AgNPs with antimicrobial proteins (AMPs).This may help to develop new and better complexes of AMP-dendronized AgNPs acting against resistant bacteria with reduced affinity to serum proteins to improve their antimicrobial properties.

抗菌素耐药性已成为全球性威胁，因此迫切需要用于治疗细菌感染的新型抗菌剂。覆盖有阳离子碳硅烷（CBS）树突的银纳米颗粒（AgNPs）是有前途的纳米材料，这是因为它们具有抗菌活性和传递抗菌剂（如抗菌蛋白（AMP））的能力。溶菌酶和噬菌体内溶素。但是，如果不了解和控制溶素与树突状AgNP之间相互作用的性质以及血清蛋白对NP表面的亲和力，则该多功能系统在医学和生物学中的应用受到限制。在本文中，我们研究了聚乙二醇化和非聚乙二醇化CBS树突化的AgNPs（分别为PEG-Dend-AgNPs和Dend-AgNPs）与KP27内溶素的相互作用，溶菌酶和人血清白蛋白（HSA），以检查NP与蛋白质之间的结合机制和亲和力以及PEG链在树状银纳米颗粒表面上的作用。结合机理的热泳，荧光，热力学，SPR和吸附分析表明，内溶素和溶菌酶对PEG-Dend-AgNPs具有更高的亲和力，而HSA对Dend-AgNPs具有更高的亲和力。溶菌酶和内溶素产生单层“蛋白质-PEG-Dend-AgNPs”电晕，HSA产生多层“蛋白质-Dend-AgNPs”。这些现象有助于我们了解表面改性如何参与树突化AgNP与抗菌蛋白（AMP）相互作用的机制。