Abstract Looking at current trends within downstream processing (DSP) of high value bioproducts, it shows that there are ongoing efforts in replacing batch processes by continuous variants. However, a unit procedure which still lacks a simple and compact continuous variant is diafiltration. Here, we present such a single piece of diafiltration equipment achieving continuous buffer exchange of up to 99.90%. The device is composed of a 3D-printed single pass diafiltration (SPDF) module containing two commercial ultrafiltration membranes. While the retentate is flowing through a narrow channel between the two membranes, the channels above and below can supply diafiltration buffer or remove permeate solution. The obtained results illustrate systematically the vulnerability of the device to the effect of concentration polarization at the membrane surface, and that this problem can be strongly reduced using an alternating direction of diafiltration buffer perfusion through the membranes as process inherent backflush. By this, a quasi-stationary operation could be obtained during continuous diafiltration, making the device an interesting option for in-process buffer exchange.

中文翻译：

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具有交替渗透流方向的连续单程渗滤，用于高效缓冲液交换

摘要 着眼于高价值生物产品下游加工 (DSP) 的当前趋势，它表明正在努力用连续变体代替批处理过程。然而，仍然缺乏简单和紧凑的连续变体的单元程序是渗滤。在这里，我们展示了这样一种单件渗滤设备，可实现高达 99.90% 的连续缓冲液交换。该设备由一个 3D 打印的单程渗滤 (SPDF) 模块组成，该模块包含两个商用超滤膜。当渗余液流过两个膜之间的狭窄通道时，上方和下方的通道可以提供渗滤缓冲液或去除渗透溶液。获得的结果系统地说明了该装置对膜表面浓差极化影响的脆弱性，并且可以使用交替方向的渗滤缓冲液灌注通过膜作为过程固有的反冲洗来大大减少这个问题。通过这种方式，可以在连续渗滤过程中获得准静态操作，使该设备成为过程中缓冲液交换的有趣选择。