In the present work, we investigated the minimal inhibitory concentration (MIC) against fungal strains (Fonsecaea pedrosoi, Microsporum canis, Candida albicans, Cryptococcus neoformans), and cytotoxicity to normal cell lines for modified red angico gum (AG) with eterifying agent N-chloride (3-chloro-2-hydroxypropyl) trimethylammonium (CHPTAC). Quaternized ammonium groups were linked to AG backbone using N-(3-chloro-2-hydroxypropyl) trimethylammonium chloride. The chemical features of the quaternized gum derivatives (QAG) were analyzed by: FTIR, elemental analysis, Zeta potential and gel permeation chromatography. The angico quaternizated gum presented a degree of substitution (DS) of 0.22 and Zeta potential of +36.43. For the antifungal test, it was observed that unmodified gum did not inhibit fungal growth. While, QAG inhibited the growth of most fungi used in this study. By AFM technique QAG interacted with the fungal surface, altering wall roughness significantly. The probable affinity of fragments of the QAG structure for the fungal enzyme 5I33 (Adenylosuccinate synthetase) has been shown by molecular docking. Low cytotoxicity was observed for polymers (unmodified gum and QAG). The results demonstrate that the quaternized polymer of AG presented in this study is a quite promising biomaterial for biotechnological applications.

在目前的工作中，我们研究了对真菌菌株（Fonsecaea pedrosoi，Microsporum canis，白色念珠菌，新隐球菌）的最小抑制浓度（MIC）。），并使用修饰剂N-氯化物（3-氯-2-羟丙基）三甲基铵（CHPTAC）对改良的红色安吉科胶（AG）对正常细胞系的细胞毒性。使用N-（3-氯-2-羟丙基）三甲基氯化铵将季铵化的铵基连接至AG主链。通过以下方法分析季铵化胶衍生物（QAG）的化学特征：FTIR，元素分析，Zeta电位和凝胶渗透色谱法。Angico季铵化口香糖的取代度（DS）为0.22，Zeta电位为+36.43。对于抗真菌试验，观察到未改性的口香糖不会抑制真菌的生长。同时，QAG抑制了这项研究中使用的大多数真菌的生长。通过AFM技术，QAG与真菌表面相互作用，显着改变壁的粗糙度。QAG结构的片段对真菌酶5I33（腺苷琥珀酸合成酶）的亲和力已通过分子对接显示。对于聚合物（未改性的口香糖和QAG）观察到低的细胞毒性。结果表明，本研究中提出的AG季铵化聚合物是一种非常有前途的生物技术生物材料。