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Comparative transcriptome analysis of Tilletia horrida infection in resistant and susceptible rice (Oryza sativa L.) male sterile lines reveals potential candidate genes and resistance mechanisms.
Genomics ( IF 4.4 ) Pub Date : 2020-09-20 , DOI: 10.1016/j.ygeno.2020.09.036
Aijun Wang 1 , Zhongping Zha 2 , Desuo Yin 3 , Xinyue Shu 1 , Li Ma 1 , Linxia Wang 4 , Ping Li 5 , Aiping Zheng 1
Affiliation  

Rice kernel smut (RKS), caused by the basidiomycete fungus Tilletia horrida, is one of the most devastating diseases affecting the production of male sterile lines of rice (Oryza sativa) worldwide. However, the molecular mechanisms of resistance to T. horrida have not yet been explored. In the present study, RNA sequencing analysis of rice male sterile lines, that are resistant and susceptible to RKS (Jiangcheng 3A and 9311A, respectively) was conducted after T. horrida infection. Transcriptomic analysis showed that a greater number of differentially expressed gene (DEGs) was observed in Jiangcheng 3A compared with 9311A after T. horrida inoculation. Furthermore, 4, 425 DEGs were uniquely detected in Jiangcheng 3A, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of these DEGs revealed that oxidoreductase activity, peroxidase activity, cutin, suberine and wax biosynthesis, and flavonoid biosynthesis were key pathways for T. horrida resistance. In summary and based on transcriptome analysis, we suggest a preliminary regulatory mechanism for Jiangcheng 3A cultivar resistance response to T. horrida inoculation.



中文翻译:

抗性和易感水稻 (Oryza sativa L.) 雄性不育系中 Tilletia horrida 感染的比较转录组分析揭示了潜在的候选基因和抗性机​​制。

稻粒黑粉病(RKS),所造成的担子菌类真菌腥黑粉horrida,是影响生产的大米的不育系(最具破坏性的疾病之一全世界)。然而,尚未探索对T. horrida抗性的分子机制。在本研究中,在T. horrida感染后对 RKS(分别为江城 3A 和 9311A)具有抗性和敏感性的水稻雄性不育系进行了 RNA 测序分析。转录组学分析表明,与 9311A 相比,在江城 3A 中观察到更多数量的差异表达基因 (DEG) 接种。此外,在江城 3A 中独特地检测到 4, 425 个 DEG,对这些 DEG 的基因本体论 (GO) 和京都基因与基因组百科全书 (KEGG) 分析揭示了氧化还原酶活性、过氧化物酶活性、角质、木栓质和蜡生物合成以及类黄酮生物合成是T. horrida抗性的关键途径。总之,基于转录组分析,我们提出了江城 3A 品种对T. horrida接种的抗性反应的初步调控机制。

更新日期:2020-09-20
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