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Construction of a novel cold-adapted oleaginous yeast consortium valued for textile azo dye wastewater processing and biorefinery
Fuel ( IF 7.4 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.fuel.2020.119050
Sameh S. Ali , Jianzhong Sun , Eleni Koutra , Nessma El-Zawawy , Tamer Elsamahy , Mohamed El-Shetehy

Abstract A new lipid-accumulating and cold-adapted oleaginous yeast consortium MG-Y-SH which stands for molecularly identified species Meyerozyma guilliermondii, Yarrowia sp. and Sterigmatomyces halophilus was successfully constructed in this study. Its total saturated fatty acid content (34.64 ± 0.95%) was higher than that of jatropha oil (21.52%). The oil obtained from oleaginous yeast consortium MG-Y-SH is advantageous for biodiesel production, since it contains low amount (21.48 ± 1.1%) of polyunsaturated fatty acids (C18:2 and C18:3). Lipase, which is a biocatalyst for the production of biodiesel by oleaginous yeasts, reached its highest specific activity of 8.35 ± 0.14 U/mg (extracellular) and 7.901 ± 0.12 U/mg (intracellular) after 36 h of incubation compared to the individual strains. Seven dye mixtures, six dyes in each group, were constructed and the maximum decolorization efficiency ranged between 55.81% (mixture III) and 80.56% (mixture VI) within 24 h of treatment with MG-Y-SH at 18 °C and under static conditions. The maximum decolorization efficiency by MG-Y-SH reached 100% at 100 mg/L Reactive Red 120 (RR120) within 3 h. Based on our investigation and analysis on those metabolites drawn from the mass spectrum as well as various induced enzymes, a possible dye biodegradation pathway linked to fatty acid synthesis was proposed. The results of phytotoxicity indicate a capability of MG-Y-SH in converting the toxic azo dye RR120 into some non-toxic metabolites, suggesting MG-Y-SH as a promising multipurpose oleaginous yeast consortium suitable for biodiesel production in the future, while degrading recalcitrant dyes and lignin valorization in cold environments.

中文翻译:

一种新型冷适应含油酵母菌群的构建,对纺织偶氮染料废水处理和生物精炼有价值

摘要 一种新的积脂和冷适应产油酵母菌 MG-Y-SH,代表分子鉴定物种 Meyerozyma guilliermondii, Yarrowia sp。本研究成功构建嗜盐Sterigmatomyces halophilus。其总饱和脂肪酸含量(34.64±0.95%)高于麻风树油(21.52%)。从含油酵母菌群 MG-Y-SH 获得的油有利于生物柴油的生产,因为它含有少量 (21.48 ± 1.1%) 的多不饱和脂肪酸 (C18:2 和 C18:3)。脂肪酶是含油酵母生产生物柴油的生物催化剂,与单个菌株相比,在孵育 36 小时后达到其最高比活性 8.35 ± 0.14 U/mg(细胞外)和 7.901 ± 0.12 U/mg(细胞内) . 七种染料混合物,每组六种染料,在 18°C 和静态条件下用 MG-Y-SH 处理 24 小时内,最大脱色效率介于 55.81%(混合物 III)和 80.56%(混合物 VI)之间。MG-Y-SH 的最大脱色效率在 100 mg/L 活性红 120 (RR120) 下在 3 小时内达到 100%。基于我们对从质谱中提取的代谢物以及各种诱导酶的调查和分析,提出了与脂肪酸合成相关的可能的染料生物降解途径。植物毒性结果表明 MG-Y-SH 能够将有毒的偶氮染料 RR120 转化为一些无毒的代谢物,表明 MG-Y-SH 是一种有前途的多用途含油酵母菌,适用于未来的生物柴油生产,同时降解在寒冷环境中顽固的染料和木质素的增值。
更新日期:2021-02-01
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