当前位置: X-MOL 学术Environ. Toxicol. Pharmacol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Enzymatic and non-enzymatic detoxification in Lycosa terrestris and Pardosa birmanica exposed to single and binary mixture of Copper and Lead.
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-09-18 , DOI: 10.1016/j.etap.2020.103500
Nida Aziz 1 , Abida Butt 1
Affiliation  

Organisms employ various enzymatic and non-enzymatic detoxification mechanisms to minimize the harmful effects of metal pollution in the terrestrial environment. We examined the effects of copper (Cu), lead (Pb) and their mixture (Cu + Pb) on glutathione (GSH), metallothionein (MTs), cytochrome P450 (CYP 450), carboxylesterase (CarbE), acetylcholinesterase (AchE) and glutathione S-transferase (GST) in Lycosa terrestris and Pardosa birmanica via two exposure routes, i.e., soil and food for 10, 20 and 40 days. The present results revealed that the accumulation of Cu and Pb in both spiders’ species increase with exposure duration and depend on the route of exposure and type of metal. The activities of CarbE, GST, and MTs significantly increased with increasing metal body burden for all experimental treatments. The CYP 450 activity exhibited a significant time-dependent decrease with increasing Cu concentration in both species. The AchE activity was significantly inhibited on Pb exposure via soil and Cu + Pb exposure via both routes. The decrease in the level of GSH was measured on Cu + Pb exposure via both routes. Thus, all these enzymatic and non-enzymatic responses are sensitive to the metals tested and could serve as early warning indicators for assessing the effects of metal pollution in these species.



中文翻译:

暴露于铜和铅的单一和二元混合物中的地衣草和百日草中的酶解毒和非酶解毒。

有机体采用各种酶促和非酶促解毒机制,以最大程度地减少金属污染在陆地环境中的有害影响。我们研究了铜(Cu),铅(Pb)及其混合物(Cu + Pb)对谷胱甘肽(GSH),金属硫蛋白(MTs),细胞色素P450(CYP 450),羧酸酯酶(CarbE),乙酰胆碱酯酶(AchE)和陆生番茄Pardosa birmanica中的谷胱甘肽S-转移酶(GST)通过两种暴露途径,即土壤和食物,持续10、20和40天。目前的研究结果表明,两种蜘蛛中铜和铅的积累量随暴露时间的延长而增加,并取决于暴露的途径和金属的类型。在所有实验处理中,CarbE,GST和MT的活性均随着金属体负荷的增加而显着增加。在这两个物种中,CYP 450活性均随铜浓度的增加而显示出明显的时间依赖性降低。AchE活性显着抑制了通过土壤暴露于铅和通过两种途径暴露于Cu + Pb。通过两种途径在铜+铅暴露下测量了谷胱甘肽水平的降低。从而,

更新日期:2020-09-20
down
wechat
bug